Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using PECAM1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit anti-Human, Mouse CD31 Polyclonal Antibody | anti-PECAM1 antibody
CD31 Polyclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
CD31; Polyclonal Antibody; CD31 Polyclonal Antibody; PECAM1; CD31 ; FLJ34100; FLJ58394; PECAM-1 ; EndoCAM ; GPIIA ; PECA1;; anti-PECAM1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Concentration
1 mg/ml (varies by lot)
Sequence Length
738
Applicable Applications for anti-PECAM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1:500-1: 2000
Immunohistochemistry (IHC): 1:50-1: 200
Immunoprecipitation (IP): 1:20-1: 50
Flow Cytometry (FC/FACS): 1:20-1: 50
Immunohistochemistry (IHC): 1:50-1: 200
Immunoprecipitation (IP): 1:20-1: 50
Flow Cytometry (FC/FACS): 1:20-1: 50
Immunogen
Recombinant protein of human CD31
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C for short-term or -80 degree C for long-term. Avoid freeze / thaw cycles
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using PECAM1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using PECAM1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Related Product Information for anti-PECAM1 antibody
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCgamma and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
References
1. Newman, P.J. (1997) J. Clin. Invest. 99, 3-8. 2. Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-15772. 3. Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-524.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
130kDa
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
NCBI Protein Information
platelet endothelial cell adhesion molecule; CD31 antigen
UniProt Protein Name
Platelet endothelial cell adhesion molecule
Protein Family
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
UniProt Entry Name
PECA1_HUMAN
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Product Notes
The PECAM1 pecam1 (Catalog #AAA125831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD31 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Western Blot (WB): 1:500-1: 2000 Immunohistochemistry (IHC): 1:50-1: 200 Immunoprecipitation (IP): 1:20-1: 50 Flow Cytometry (FC/FACS): 1:20-1: 50. Researchers should empirically determine the suitability of the PECAM1 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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