Mouse Galectin 9 (GAL9) CLIA Kit | GAL9 clia kit

Mouse Galectin 9 (GAL9) CLIA Kit

Cat. Num. AAA2708745

• Gene Names: Lgals9; gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Galectin 9 (GAL9); Mouse Galectin 9 (GAL9) CLIA Kit; Galectin 9; LGALS9; Ecalectin; Lectin; Galactoside-Binding Soluble 9; Tumor antigen HOM-HD-21; GAL9 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Galectin 9 (GAL9).; No significant cross-reactivity or interference between Galectin 9 (GAL9) and analogues was observed.
• Sequence Length: 322

• Applicable Applications for GAL9 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.8pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Galectin 9 (GAL9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Galectin 9 (GAL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Galectin 9 (GAL9) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 226531139
• NCBI GeneID: 16859
• NCBI Accession #: NP_001152773.1
• NCBI GenBank Nucleotide #: NP_001152773.1
• UniProt Accession #: O08573
• NCBI Official Full Name: galectin-9 isoform 2
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 9
• NCBI Official Symbol: Lgals9
• NCBI Official Synonym Symbols: gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
• NCBI Protein Information: galectin-9
• UniProt Protein Name: Galectin-9
• Protein Family: Gallinacin
• UniProt Gene Name: Lgals9
• UniProt Synonym Gene Names: Gal-9
• UniProt Entry Name: LEG9_MOUSE

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Cellular Component: cytoplasm; extracellular region

Molecular Function: galactoside binding; carbohydrate binding

Biological Process: heterophilic cell adhesion