LGALS9 recombinant protein

LGALS9, 1-322aa, Mouse, His tag, E Coli (Denatured)

Cat. Num. AAA205079

• Gene Names: Lgals9; gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: LGALS9; 1-322aa; Mouse; His tag; E Coli (Denatured); Galectin-9; gal-9; galectin-9; LGALS35; Lgals5; gal9; LGALS9 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMALFSAQ SPYINPIIPF TGPIQGGLQE GLQVTLQGTT KSFAQRFVVN FQNSFNGNDI AFHFNPRFEE GGYVVCNTKQ NGQWGPEERK MQMPFQKGMP FELCFLVQRS EFKVMVNKKF FVQYQHRVPY HLVDTIAVSG CLKLSFITFQ TQNFRPAHQA PMAQTTIHMV HSTPGQMFST PGIPPVVYPT PAYTIPFYTP IPNGLYPSKS IMISGNVLPD ATRFHINLRC GGDIAFHLNP RFNENAVVRN TQINNSWGQE ERSLLGRMPF SRGQSFSVWI ICEGHCFKVA VNGQHMCEYY HRLKNLQDIN TLEVAGDIQL THVQT
• Sequence Length: 322

• Applicable Applications for LGALS9 recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LGALS9 recombinant protein

LGALS9 is a member of animal lectins that have an affinity to beta-galactosides and forssman pentasaccharide. It is strongly overexpressed in Hodgkin's disease tissue and it might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its consistently associated immunodeficiency. Recombinant mouse LGALS9 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for LGALS9 recombinant protein

Cell Cycle

References

Hirashima M, Kashio Y, et al. (2004). Glycoconj J. (7-9):593-600.; Muehlfeit M, et al. (2006). Biochem Biophys Res Commun. 351 (2):571-6.

NCBI and Uniprot Product Information

• NCBI GI #: 226531139
• NCBI GeneID: 16859
• NCBI Accession #: NP_001152773
• NCBI GenBank Nucleotide #: NM_001159301.1
• UniProt Accession #: O08573; O08572; Q3UKE5; Q99L83
• Molecular Weight: 38kDa (345aa)
• NCBI Official Full Name: galectin-9 isoform 2
• NCBI Official Synonym Full Names: lectin, galactose binding, soluble 9
• NCBI Official Symbol: Lgals9
• NCBI Official Synonym Symbols: gal-9; Lgals5; LGALS35; AA407335; AI194909; AI265545; galectin-9
• NCBI Protein Information: galectin-9
• UniProt Protein Name: Galectin-9
• Protein Family: Galectin
• UniProt Gene Name: Lgals9
• UniProt Synonym Gene Names: Gal-9

Uniprot Description

Binds galactosides (). Has high affinity for the Forssman pentasaccharide (). Ligand for HAVCR2/TIM3 (). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth (PubMed:20937702). Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307). Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (PubMed:25065622). Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation (). Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection (). Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells (). Inhibits degranulation and induces apoptosis of mast cells (). Induces maturation and migration of dendritic cells (). Inhibits natural killer (NK) cell function (PubMed:23408620). Can transform NK cell phenotype from peripheral to decidual during pregnancy (). Astrocyte derived galectin-9 enhances microglial TNF production (PubMed:25158758). May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion (). Highly selective to the anion urate ().