Western Blot (WB)
(All lanes: HepG2 Whole Cell Lysate (40ug);Lane 1.: Anti-GAPDH antibody ;Lane 2.: Anti-Beta tubulin antibody )
HepG2 cell lysate
HepG2
Applications
Western Blot
Synonyms
HepG2; HepG2 cell lysate
Host
Human
Form/Format
Liquid; In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue).
Concentration
2mg/ml (determined by BCA assay) (varies by lot)
Applicable Applications for HepG2 cell lysate
Western Blot (WB)
Quality Control
Western Blot analyzed by both GAPDH and Beta tubulin antibody .
Preparation Before Usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Preparation and Storage
Aliquot the lysate into single tube and then storing them -80 degree C or below. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(All lanes: HepG2 Whole Cell Lysate (40ug);Lane 1.: Anti-GAPDH antibody ;Lane 2.: Anti-Beta tubulin antibody )
Western Blot (WB)
(All lanes: HepG2 Whole Cell Lysate (40ug);Lane 1.: Anti-GAPDH antibody ;Lane 2.: Anti-Beta tubulin antibody )
Related Product Information for HepG2 cell lysate
HepG2 cells are immortalized cell line that derived from liver tissue of a 15-year-old boy with hepatocellular carcinoma. HepG2 cells secrete a variety of major plasma proteins such as albumin, transferrin, and secrete acute-phase proteins such as fibrinogen, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin, and plasminogen. HepG2 have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies.
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Product Notes
The HepG2 (Catalog #AAA201020) is a Cell Lysate produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HepG2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the HepG2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HepG2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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