Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Hydroxyacid Oxidase 1 (HAO1) Polyclonal Antibody | anti-HAO1 antibody
Polyclonal Antibody to Hydroxyacid Oxidase 1 (HAO1)
Gene Names
Hao1; Gox1; XDH1
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Hydroxyacid Oxidase 1 (HAO1); Polyclonal Antibody; Polyclonal Antibody to Hydroxyacid Oxidase 1 (HAO1); anti-HAO1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HAO1. It has been selected for its ability to recognize HAO1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GTGMMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREVS SQLVKRAEQM GYKAIFVTVD TPYLGNRFDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG DNSGLAEYVA QAIDPSLSWD DIKWLRRLTS LPIVVKGILR GDDAQEAVKH GVDGILVSNH GARQLDGVPA TIDALPEIVE AVEGKVEVFL DGGVRKGTDV LKALALGARA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ NVKVIDK
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GTGMMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREVS SQLVKRAEQM GYKAIFVTVD TPYLGNRFDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG DNSGLAEYVA QAIDPSLSWD DIKWLRRLTS LPIVVKGILR GDDAQEAVKH GVDGILVSNH GARQLDGVPA TIDALPEIVE AVEGKVEVFL DGGVRKGTDV LKALALGARA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ NVKVIDK
Sequence Length
370
Applicable Applications for anti-HAO1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant HAO1 (Gly102~Lys357) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2077931
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,968 Da
NCBI Official Full Name
hydroxyacid oxidase 1
NCBI Official Synonym Full Names
hydroxyacid oxidase 1
NCBI Official Symbol
Hao1
NCBI Official Synonym Symbols
Gox1; XDH1
NCBI Protein Information
hydroxyacid oxidase 1
UniProt Protein Name
Hydroxyacid oxidase 1
Protein Family
UniProt Gene Name
Hao1
NCBI Description
catalyzes the oxidation of glycolate to glyoxylate and H2O2; may be involved in response to oxidative stress [RGD, Feb 2006]
Uniprot Description
HAO1: Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2- hydroxy octanoate. Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.3.15; Oxidoreductase
Chromosomal Location of Human Ortholog: 3q36
Cellular Component: peroxisome
Molecular Function: (S)-2-hydroxy-acid oxidase activity; FMN binding; glycolate oxidase activity; receptor binding
Biological Process: fatty acid alpha-oxidation; glycolate catabolic process; response to oxidative stress
Research Articles on HAO1
Similar Products
Product Notes
The HAO1 hao1 (Catalog #AAA2001496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Hydroxyacid Oxidase 1 (HAO1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hydroxyacid Oxidase 1 (HAO1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HAO1 hao1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GTG MMLSSW ATSSIEEVAE AGPEALRWMQ LYIYKDREVS SQLVKRAEQM GYKAIFVTVD TPYLGNRFDD VRNRFKLPPQ LRMKNFETND LAFSPKGNFG DNSGLAEYVA QAIDPSLSWD DIKWLRRLTS LPIVVKGILR GDDAQEAVKH GVDGILVSNH GARQLDGVPA TIDALPEIVE AVEGKVEVFL DGGVRKGTDV LKALALGARA VFVGRPIIWG LAFQGEKGVQ DVLEILKEEF RLAMALSGCQ NVKVIDK. It is sometimes possible for the material contained within the vial of "Hydroxyacid Oxidase 1 (HAO1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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