BMP10 active protein

Bmp10, Recombinant, Mouse (Bone Morphogenetic Protein 10, BMP-10)

Cat. Num. AAA650453

• Gene Names: BMP10; MGC126783
• Reactivity: Mouse
• Purity: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Synonyms: Bmp10; Recombinant; Mouse (Bone Morphogenetic Protein 10; BMP-10); BMP10 active protein

• Reactivity: Mouse
• Purity/Purification: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Form/Format: Supplied as a lyophilized powder from HCl, BSA. Reconstitute with 4mM HCl, 0.1% human or bovine serum albumin.

• Application Notes: SDS-PAGE: 10kD, reducing conditions
• Activity: Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 15-75ng/ml.
• Endotoxin Level: 1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with 4mM HCl containing at least 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for BMP10 active protein

Bone morphogenetic protein 10 (BMP-10), along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-b superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. Proteolytic removal of the propeptide from the 60kD proprotein yields a 12kD mature BMP-10 which forms disulfide-linked nonglycosylated homodimers. In transfectants, BMP-10 can also be secreted as a proprotein which binds to matrix fibrillin. Mature mouse BMP-10 shares 98% and 100ªsequence identity with human and rat BMP-10, respectively, and 48%-64% with mouse BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation. Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development. BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the Notch-dependent growth of embryonic cardiomyocytes. NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes. BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size and is upregulated in hypertrophied ventricles. Mature BMP-10 accumulates within cardiomyocytes in association with the sarcomere protein Tcap. BMP-10 induces signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines. Deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities. In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1.

Product Categories/Family for BMP10 active protein

Growth Factors; Cytokines; Growth Factors-BMP

NCBI and Uniprot Product Information

• NCBI GI #: 7656928
• NCBI GeneID: 27302
• NCBI Accession #: NP_055297.1
• NCBI GenBank Nucleotide #: NM_014482.1
• UniProt Accession #: O95393; Q53R17; Q6NTE0
• Molecular Weight: ~12kD
• NCBI Official Full Name: bone morphogenetic protein 10 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 10
• NCBI Official Symbol: BMP10
• NCBI Official Synonym Symbols: MGC126783
• NCBI Protein Information: bone morphogenetic protein 10; BMP-10; OTTHUMP00000159973
• UniProt Protein Name: Bone morphogenetic protein 10
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP10
• UniProt Entry Name: BMP10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta family of growth factors. Data suggest that the similar protein in mouse plays an important role in trabeculation of the embryonic heart. In human, this protein may signal through receptor serine/threonine kinases. [provided by RefSeq]

Uniprot Description

Function: Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. Ref.4 Ref.5

Subunit structure: Homodimer; disulfide-linked

Subcellular location: Secreted

Sequence similarities: Belongs to the TGF-beta family.