Immunofluorescence/Immunocytochemistry (IF/ICC)
Mouse anti-Xenopus Y14 Monoclonal Antibody | anti-Y14 antibody
Mouse Anti-Y14 [4C4]
Reactivity
Xenopus
Applications
ELISA
Purity
Protein G
Synonyms
Y14; Monoclonal Antibody; Mouse Anti-Y14 [4C4]; Binder of OVCA1 1 antibody; Binder of OVCA1-1 antibody; BOV 1 antibody; BOV 1A antibody; BOV 1Bantibody; BOV 1C antibody; BOV-1 antibody; BOV-1A antibody; BOV-1B antibody; BOV-1C antibody; BOV1antibody; BOV1A antibody; BOV1B antibody; BOV1C antibody; HSPC 114 antibody; HSPC114 antibody; MDS 014antibody; MDS014 antibody; RBM 8 antibody; RBM 8A antibody; RBM 8B antibody; RBM8 antibody; rbm8aantibody; RBM8A_HUMAN antibody; RBM8B antibody; Ribonucleoprotein RBM 8 antibody; Ribonucleoprotein RBM 8Aantibody; Ribonucleoprotein RBM8 antibody; Ribonucleoprotein RBM8A antibody; RNA binding motif protein 8antibody; RNA binding motif protein 8A antibody; RNA binding motif protein 8B antibody; RNA binding protein 8Aantibody; RNA binding protein Y14 antibody; RNA-binding motif protein 8A antibody; RNA-binding protein 8Aantibody; RNA-binding protein Y14 antibody; ZNRP antibody; ZRNP 1 antibody; ZRNP1 antibody; anti-Y14 antibody
Host
Mouse
Reactivity
Xenopus
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
4C4
Specificity
Human Y14. Detects a band of approximately 22 kDa .
Purity/Purification
Protein G
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Y14 antibody
ELISA, western blot, immunoprecipitation & immunofluorescence
Application Notes
Optimal antibody diluiton should be determined by titration, however as a guideline try at 1:100 for western blot (HeLa extracts).
Target Antigen
Y14
Immunogen
Full length purified Y14 protein(Human).
Note
Product that contains sodium azide (a posinous and hazardous substance) should be handled by trained staff only.
Myeloma/fusion Partners
Balb/c Sp2/0 cell line
Research Areas
Chromatin; Regulation; RNA binding; Splicing
Preparation and Storage
Store at 4°C for one month, or in small aliquots at -20°C for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Immunofluorescence/Immunocytochemistry (IF/ICC)
Immunofluorescence/Immunocytochemistry (IF/ICC)
References
Shigeoka T et al. Evidence that the Upf1-related molecular motor scans the 3'-UTR to ensure mRNA integrity.Nucleic Acids Res. 2012 Aug;40(14):6887-97. doi: 10.1093/nar/gks344. Epub 2012 May 2.WB ; Human PMID: 22554850 Stroupe ME et al. The three-dimensional arcitecture of the EJC core.J Mol Biol. 2006 Jul 21;360(4):743-9. Epub 2006 Jun 5.WB ; Human PMID: 16797590 PremRNA splicing imprints mRNA in the nucleus with a novel RNAbinding protein that persists in the cytoplasm. Kataoka N (Molecular and Cellular Biology,Sep 2000) PUBMED
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Product Notes
The Y14 (Catalog #AAA375111) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Y14 [4C4] reacts with Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Y14 can be used in a range of immunoassay formats including, but not limited to, ELISA, western blot, immunoprecipitation & immunofluorescence. Optimal antibody diluiton should be determined by titration, however as a guideline try at 1:100 for western blot (HeLa extracts). Researchers should empirically determine the suitability of the Y14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Y14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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