Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant WNT7B, Human)
Mouse anti-Human Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) Monoclonal Antibody | anti-WNT7B antibody
Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 7B (WNT7B)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Wingless Type MMTV Integration Site Family; Member 7B (WNT7B); Monoclonal Antibody; Monoclonal Antibody to Wingless Type MMTV Integration Site Family; anti-WNT7B antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C4
Specificity
The antibody is a mouse monoclonal antibody raised against WNT7B. It has been selected for its ability to recognize WNT7B in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Thr216~Lys349 with Two N-terminal Tags, His-tag and SUMO-tag
Sequence Length
349
Applicable Applications for anti-WNT7B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:500-2000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant WNT7B, Human)
Western Blot (WB)
(Figure. Western Blot: Sample: Recombinant WNT7B, Human)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein. [provided by RefSeq, Oct 2008]
Research Articles on WNT7B
Similar Products
Product Notes
The WNT7B (Catalog #AAA2111332) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Wingless Type MMTV Integration Site Family, Member 7B (WNT7B) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:500-2000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the WNT7B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Thr216~Lys 349 with Two N-terminal Tags, His-tag and SUMO-tag. It is sometimes possible for the material contained within the vial of "Wingless Type MMTV Integration Site Family, Member 7B (WNT7B), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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