Rabbit anti-Mouse, rat Med15 Polyclonal Antibody | anti-Med15 antibody

(Mouse) Med15 Antibody (C-term)

Cat. Num. AAA9202903

• Reactivity: Mouse, rat
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Med15; Polyclonal Antibody; (Mouse) Med15 Antibody (C-term); Mediator of RNA polymerase II transcription subunit 15; Mediator complex subunit 15; Positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; mPcqap; Pcqap; anti-Med15 antibody

• Host: Rabbit
• Reactivity: Mouse, rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This mouse Med15 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 712-746 amino acids from the C-terminal region of mouse Med15.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 792

• Applicable Applications for anti-Med15 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 87 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-Med15 Antibody (C-term) at 1:2000 dilutionLane 1: mouse brain lysatesLane 2: mouse spleen lysatesLane 3: mouse thymus lysatesLane 4: rat lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 87 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Med15 antibody

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).

Product Categories/Family for anti-Med15 antibody

Crown Antibodies

References

Berti L.,et al.Genomics 74:320-332(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 20177999
• UniProt Accession #: Q924H2
• Molecular Weight: 87061
• NCBI Official Full Name: Mediator of RNA polymerase II transcription subunit 15
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 15
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: Med15
• UniProt Synonym Gene Names: Pcqap; PC2 glutamine/Q-rich-associated protein; mPcqap
• UniProt Entry Name: MED15_MOUSE

Uniprot Description

PCQAP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. Belongs to the Mediator complex subunit 15 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Cellular Component: cytoplasm; Srb-mediator complex; nucleus

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; stem cell maintenance