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Western Blot (WB)
(Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M Lane 2: U937 Lane 3: SH-SY-5Y)
Rabbit anti-Human, Mouse VPS35 Monoclonal Antibody | anti-VPS35 antibody
VPS35 Antibody
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
VPS35; Monoclonal Antibody; VPS35 Antibody; DKFZp434E1211 antibody; DKFZp434P1672 antibody; FLJ10752 antibody; FLJ13588 antibody; FLJ20388 antibody; hVPS35 antibody; Maternal embryonic 3 antibody; Maternal-embryonic 3 antibody; MEM 3 antibody; MEM3 antibody; PARK17 antibody; TCCCTA00141 antibody; Vacuolar protein sorting 35 (yeast) antibody; Vacuolar protein sorting 35 antibody; Vacuolar protein sorting 35 homolog antibody; Vacuolar protein sorting associated protein 35 antibody; Vacuolar protein sorting-associated protein 35 antibody; Vesicle protein sorting 35 antibody; VPS 35 antibody; VPS35 antibody; VPS35_HUMAN antibody; anti-VPS35 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
JB33-82
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-VPS35 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:300
FC/FACS: 1:50-1:100
IHC: 1:100-1:300
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M Lane 2: U937 Lane 3: SH-SY-5Y)
Western Blot (WB)
(Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M Lane 2: U937 Lane 3: SH-SY-5Y)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-VPS35 antibody
Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS35 (vacuolar protein sorting 35), also known as MEM3, is the 796 amino acid human homolog of the S. cerevisiae Vps35 protein. Localized to the cytoplasm and to the peripheral membrane, VPS35 is an essential component of the retromer complex which is involved in retrieval of lysosomal enzyme receptors from endosomes to the trans-Golgi network. VPS35 is expressed ubiquitously with highest expression in heart, placenta, brain, testis, kidney, colon, ovary and spleen. In addition to its crucial role in the retromer complex, VPS35 is part of a subcomplex that is required to regulate transcytosis of the polymeric immunoglobulin receptor from the basolateral to the apical surface of epithelial cells and hepatocytes.
Product Categories/Family for anti-VPS35 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,707 Da
NCBI Official Full Name
vacuolar protein sorting-associated protein 35
NCBI Official Synonym Full Names
vacuolar protein sorting 35 homolog (S. cerevisiae)
NCBI Official Symbol
VPS35
NCBI Official Synonym Symbols
MEM3; PARK17
NCBI Protein Information
vacuolar protein sorting-associated protein 35; hVPS35; maternal-embryonic 3
UniProt Protein Name
Vacuolar protein sorting-associated protein 35
Protein Family
UniProt Gene Name
VPS35
UniProt Synonym Gene Names
MEM3; hVPS35
UniProt Entry Name
VPS35_HUMAN
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Product Notes
The VPS35 vps35 (Catalog #AAA9436524) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS35 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VPS35 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:100-1:300 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the VPS35 vps35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS35, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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