Rabbit anti-Human VPS35 Polyclonal Antibody | anti-VPS35 antibody

VPS35 Antibody

Cat. Num. AAA7046183

• Gene Names: VPS35; MEM3; PARK17
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: VPS35; Polyclonal Antibody; VPS35 Antibody; Vacuolar protein sorting-associated protein 35; hVPS35; Maternal-embryonic 3; Vesicle protein sorting 35; MEM3; TCCCTA00141; anti-VPS35 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 796

• Applicable Applications for anti-VPS35 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: IHC: 1:20-1:200
• Species: Human
• Immunogen: Recombinant human Vacuolar protein sorting-associated protein 35 protein (N-250AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-55803 / sc-55805
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7046183 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis using MBS7046183 at dilution 1:100)

Related Product Information for anti-VPS35 antibody

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15078903, PubMed:15247922, PubMed:20164305). Required for endosomal localization of FAM21C (PubMed:22070227). Mediates the association of the CSC with the WASH complex via FAM21 (PubMed:22070227, PubMed:24980502, PubMed:24819384). Required for the endosomal localization of TBC1D5 (PubMed:20923837).

NCBI and Uniprot Product Information

• NCBI GI #: 17999541
• NCBI GeneID: 55737
• NCBI Accession #: NP_060676.2
• NCBI GenBank Nucleotide #: NM_018206.5
• UniProt Accession #: Q96QK1; Q561W2; Q9H016; Q9H096; Q9H4P3; Q9H8J0; Q9NRS7; Q9NVG2; Q9NX80; Q9NZK2
• Molecular Weight: 91,707 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 35
• NCBI Official Synonym Full Names: VPS35, retromer complex component
• NCBI Official Symbol: VPS35
• NCBI Official Synonym Symbols: MEM3; PARK17
• NCBI Protein Information: vacuolar protein sorting-associated protein 35
• UniProt Protein Name: Vacuolar protein sorting-associated protein 35
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS35
• UniProt Synonym Gene Names: MEM3; hVPS35
• UniProt Entry Name: VPS35_HUMAN

NCBI Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS35: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Defects in VPS35 are the cause of Parkinson disease type 17 (PARK17). PARK17 is an autosomal dominant, adult- onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. Belongs to the VPS35 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 16q12

Cellular Component: cytosol; early endosome; endosome; late endosome; lysosomal membrane; retromer complex

Molecular Function: protein binding; protein transporter activity

Biological Process: intracellular protein transport; regulation of macroautophagy; regulation of protein stability; retrograde transport, endosome to Golgi; transcytosis; Wnt receptor signaling pathway

Disease: Parkinson Disease 17