Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) Standard Curve.)
Rat Visceral Adipose-Specific Serine Protease Inhibitor (SERPINA12) ELISA Kit | SERPINA12 elisa kit
Rat Visceral Adipose-Specific Serine Protease Inhibitor (SERPINA12) ELISA Kit
Reactivity
Rat
Synonyms
Visceral Adipose-Specific Serine Protease Inhibitor (SERPINA12); Rat Visceral Adipose-Specific Serine Protease Inhibitor (SERPINA12) ELISA Kit; SERPINA12; OL-64; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 12; vaspin; visceral adipose-specific SERPIN; SERPINA12 elisa kit
Reactivity
Rat
Specificity
Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPINA12. No significant cross-reactivity or interference between Rat SERPINA12 and analogues was observed.
Sequence Length
411
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) Standard Curve.)
Related Product Information for SERPINA12 elisa kit
Description: This Rat Visceral adipose-specific serine protease inhibitor (SERPINA12) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA12 in samples. An antibody specific for SERPINA12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINA12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an interesting novel adipocytokine with insulin-sensitizing effects. Some studies have suggested that vaspin could play an important role in the development of obesity and metabolic disorders. The induction of vaspin mRNA expression could represent a compensatory mechanism associated with obesity, severe insulin resistance and type 2 diabetes mellitus, however it is unclear whether a correlation exists between human vaspin serum levels and markers of insulin sensitivity and glucose or lipid metabolism. Vaspin serum concentrations have been shown to be lower in lean subjects and competitive sportsmen with long-term physical training, but they are increased with weight loss associated with a physical training programme.
Background: Visceral adipose tissue-derived serine protease inhibitor (vaspin) is an interesting novel adipocytokine with insulin-sensitizing effects. Some studies have suggested that vaspin could play an important role in the development of obesity and metabolic disorders. The induction of vaspin mRNA expression could represent a compensatory mechanism associated with obesity, severe insulin resistance and type 2 diabetes mellitus, however it is unclear whether a correlation exists between human vaspin serum levels and markers of insulin sensitivity and glucose or lipid metabolism. Vaspin serum concentrations have been shown to be lower in lean subjects and competitive sportsmen with long-term physical training, but they are increased with weight loss associated with a physical training programme.
NCBI and Uniprot Product Information
Uniprot Description
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
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Product Notes
The Rat SERPINA12 serpina12 (Catalog #AAA9711895) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711895 ELISA Kit recognizes Rat SERPINA12. It is sometimes possible for the material contained within the vial of "Visceral Adipose-Specific Serine Protease Inhibitor (SERPINA12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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