Immunocytochemistry (ICC)
(Confocal fluorescent analysis of HUVEC cells using Anti-VE-cadherin (Red). Actin filaments have been labeled with Alexa Fluor488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.)
Mouse VE Cadherin Monoclonal Antibody | anti-VE antibody
VE Cadherin antibody
IHC-P: 1:10-1:20
User optimization required for FC and WB.
IHC: Buffered formalin fixation is recommended with a fixation period of < 12 hours. High heat antigen retrieval in citrate buffer is suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells. FC: Use in the presence of Ca ++. PBS/2-5mM EDTA is recommended for cell detachment. WB: Non-reducing conditions may be needed, 2-5 mM Ca++ required in buffer.
Immunocytochemistry (ICC)
(Confocal fluorescent analysis of HUVEC cells using Anti-VE-cadherin (Red). Actin filaments have been labeled with Alexa Fluor488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.)
Immunocytochemistry (ICC)
(Confocal fluorescent analysis of HUVEC cells using Anti-VE-cadherin (Red). Actin filaments have been labeled with Alexa Fluor488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cell membrane.)
Western Blot (WB)
(HUVEC cell lysate was probed with VE-cadherin antibody (1/2000 dilution).)
Western Blot (WB)
(HUVEC cell lysate was probed with VE-cadherin antibody (1/2000 dilution).)
NCBI and Uniprot Product Information
NCBI Description
This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: adherens junction; tight junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction
Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding
Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation
Research Articles on VE
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Product Notes
The VE cdh5 (Catalog #AAA832525) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's VE Cadherin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB). ICC: 1:500 IHC-P: 1:10-1:20 User optimization required for FC and WB. IHC: Buffered formalin fixation is recommended with a fixation period of < 12 hours. High heat antigen retrieval in citrate buffer is suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells. FC: Use in the presence of Ca ++. PBS/2-5mM EDTA is recommended for cell detachment. WB: Non-reducing conditions may be needed, 2-5 mM Ca++ required in buffer. Researchers should empirically determine the suitability of the VE cdh5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VE Cadherin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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