Sheep VE-Cadherin ELISA Kit | VE-Cadherin elisa kit

Sheep VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

Cat. Num. AAA2506196

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Sheep
• Synonyms: VE-Cadherin; Sheep VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit; VE-Cadherin elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep VE-Cadherin. No significant cross-reactivity or interference between Sheep VE-Cadherin and analogues was observed.
• Sequence Length: 784

• Samples: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: The minimum detectable dose of Sheep VE-Cadherin is 0.375ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep VE-Cadherin concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VE-Cadherin elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VE-Cadherin. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VE-Cadherin. You can calculate the concentration of VE-Cadherin in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 599834
• NCBI GeneID: 1003
• UniProt Accession #: P33151; Q4VAI5; Q4VAI6
• Molecular Weight: 74,499 Da
• NCBI Official Full Name: VE-cadherin
• NCBI Official Synonym Full Names: cadherin 5, type 2 (vascular endothelium)
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5; 7B4 antigen; VE-cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin
• UniProt Protein Name: Cadherin-5
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: tight junction; adherens junction; membrane; integral to membrane; plasma membrane; intercellular junction; cell junction

Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: negative regulation of cell proliferation; intercellular junction assembly and maintenance; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation