Mouse VCX-A Monoclonal Antibody | anti-VCX3A antibody

VCX-A (Variable charge X-linked protein 3, Variably charged protein X-A, VCX-8r, Variable charge protein on X with eight repeats, VCX3A, VCX3, VCX8R, VCXA)

Cat. Num. AAA605618

• Gene Names: VCX3A; VCX3; VCXA; VCX-A; VCX8R; VCX-8r
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: VCX-A; Monoclonal Antibody; VCX-A (Variable charge X-linked protein 3; Variably charged protein X-A; VCX-8r; Variable charge protein on X with eight repeats; VCX3A; VCX3; VCX8R; VCXA); Anti -VCX-A (Variable charge X-linked protein 3; anti-VCX3A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G460
• Specificity: Recognizes VCX-A. Species Crossreactivity: human, mouse, and rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-VCX3A antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.
• Immunogen: Partial recombinant human VCX-A (aa2-100) with a GST tag.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot detection of VCX-A expression in 293T transfected cell lysate (lane 1) and non-tranfected 293T lysate (lane 2).)

Related Product Information for anti-VCX3A antibody

VCX-A is only expressed in testis and may play a role in spermatogenesis or in sex ratio distortion. It is a 186aa peptide that has an estimated molecular weight of ~20kD. It was recently identified that VCX-A is an inhibitor of the decapping enzyme DCP2 and that it stabilized mRNA. It is a noncanonical cap-binding protein that binds to capped RNA but not cap structures lacking RNA. Defects in VCX-A are associated with some forms of X-linked nonspecific mental retardation.

Product Categories/Family for anti-VCX3A antibody

Antibodies; Abs to RNA Processing

NCBI and Uniprot Product Information

• NCBI GI #: 48762947
• NCBI GeneID: 51481
• NCBI Accession #: NP_057463.2
• NCBI GenBank Nucleotide #: NM_016379.3
• UniProt Accession #: Q9NNX9; Q9P0H4
• Molecular Weight: 20,020 Da
• NCBI Official Full Name: variable charge X-linked protein 3
• NCBI Official Synonym Full Names: variable charge, X-linked 3A
• NCBI Official Symbol: VCX3A
• NCBI Official Synonym Symbols: VCX3; VCXA; VCX-A; VCX8R; VCX-8r
• NCBI Protein Information: variable charge X-linked protein 3; variably charged X-A; variably charged protein X-A; variable charge protein on X with eight repeats
• UniProt Protein Name: Variable charge X-linked protein 3
• Protein Family: Variable charge X-linked protein
• UniProt Gene Name: VCX3A
• UniProt Synonym Gene Names: VCX3; VCX8R; VCXA; VCX-8r; VCX-A
• UniProt Entry Name: VCX3_HUMAN

NCBI Description

This gene belongs to the VCX/Y gene family, which has multiple members on both X and Y chromosomes, and all are expressed exclusively in male germ cells. The X-linked members are clustered on chromosome Xp22 and Y-linked members are two identical copies of the gene within a palindromic region on Yq11. The family members share a high degree of sequence identity, with the exception that a 30-bp unit is tandemly repeated in X-linked members but occurs only once in Y-linked members. The VCX gene cluster is polymorphic in terms of copy number; different individuals may have a different number of VCX genes. VCX/Y genes encode small and highly charged proteins of unknown function. The presence of a putative bipartite nuclear localization signal suggests that VCX/Y members are nuclear proteins. This gene contains 8 repeats of the 30-bp unit. [provided by RefSeq, Jul 2008]

Uniprot Description

VCX3A: May mediate a process in spermatogenesis or may play a role in sex ratio distortion. Belongs to the VCX/VCY family.

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: nucleolus; nucleus

Biological Process: brain development