Immunofluorescence (IF)
( Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody TK8-14. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of TK8-14 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody)
VAP-1 recombinant antibody
Anti-VAP-1 [TK8-14]
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
PurifiedProtein A Affinity Purified
Synonyms
VAP-1; Monoclonal Recombinant Antibody; Anti-VAP-1 [TK8-14]; VAP1; Copper amine oxidase; HPAO; Membrane primary amine oxidase; Semicarbazide-sensitive; AOC3; Amine oxidase; copper containing 3; amine oxidase; Vascular adhesion protein 1; SSAO; copper-containing amine:oxygen oxidoreductase; VAP-1 recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
TK8-14
Specificity
Recognizes human Vascular Adhesion Protein-1 (VAP-1), a glycosylated homodimericmembrane protein consisting of two 90 kDa subunits connected by disulfide bonds. Epitoperecognized by TK8-18 is present both in dimeric and monomeric forms of VAP-1. Elevated solubleVAP-1 serum- levels have been described in several inflammatory- diseases as well as colorectalcancer. TK8-14 is a function-blocking mAb.
Purity/Purification
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Applicable Applications for VAP-1 recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
Affinity purified VAP-1 from tonsil stroma of human origin.
Antigen
VAP-1
Antigen Description
Affinity purified VAP-1 from tonsil stroma of human origin.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Publication Note
Describes the production of monoclonal antibody against VAP-1 (TK8-14) and its use as a detection antibody, in studying serum level of soluble VAP-1, in a sandwich ELISA.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
( Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody TK8-14. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of TK8-14 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody)
Immunofluorescence (IF)
( Immunofluoresence staining of HeLa cells with anti-VAP-1 antibody TK8-14. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with the chimeric rabbit version of TK8-14 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody)
Related Product Information for VAP-1 recombinant antibody
Recombinant monoclonal antibody to VAP-1. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the hybridoma TK8-14.
Product Categories/Family for VAP-1 recombinant antibody
References
Kurkijärvi R, Adams DH, Leino R, Möttönen T, Jalkanen S, Salmi M. J Immunol. 1998 Aug1;161(3):1549-57. PMID:9686623
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Product Notes
The VAP-1 (Catalog #AAA488224) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-VAP-1 [TK8-14] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VAP-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the VAP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAP-1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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