Mouse anti-Human uPAR Monoclonal Antibody | anti-uPAR antibody
uPAR (D2 domain), Human, mAb IIIB11
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Synonyms
uPAR; Monoclonal Antibody; uPAR (D2 domain); Human; mAb IIIB11; anti-uPAR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Form/Format
1 ml (100 mug/ml) 0.2 mum filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-uPAR antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
Application Notes
The typical starting working dilution is 1:10.
Preparation and Storage
Should be stored at 4 degree C.
Under recommended storage conditions, product is stable for at least one year.
Under recommended storage conditions, product is stable for at least one year.
Related Product Information for anti-uPAR antibody
Monoclonal antibody IIIB11 recognizes the urokinase-type plasminogen activator receptor (uPAR or CD87), a GPI anchored highly glycosylated, single-chain polypeptide. CD87, a 45- to 60-kD protein, consists of three structurally homologous domains of approximately 90 amino acids, designated D1, D2, and D3, each containing a conserved arrangement of disulfide bonds and separated by short interdomain linker sequences. uPAR exists in various molecular forms; heavily glycosylated, a cleaved form, a soluble form and spliced forms. CD87 is expressed by monocytes/macrophages, neutrophils, endothelial, smooth muscle cells and epithelial cells. CD87 is overexpressed upon exposure to inflammatory mediators. The protein is also highly expressed by tumor cells and contributes to their invasiveness and metastatic potential. CD87 is a multiligand receptor that operates as a key element in pathophysiological processes involving cell migration and tissue remodelling during inflammation and cancer metastasis. CD87 binds with high affinity the Ser-proteinase urokinase-type plasminogen activator (uPA) which, upon binding catalyzes the conversion of plasminogen into plasmin which activates various matrix metalloproteinase leading to a high potential for pericellular proteolysis. CD87 functions also as an adhesion molecule by binding vitronectin, thus contributing to leukocytic adherence. High-affinity binding of CD87 to both uPA and vitronectin requires the presence of domain 1 (D1). Yet, CD87 participates in cell adherence and migration also through two other routes, a physical association and functional interaction with various integrins and via an intrinsic chemotactic activity associated to the D1-D2 linker sequence. Monoclonal antibody IIIB11 recognizes D2 of the urokinase-type plasminogen activator receptor (CD87). The antibody recognizes amino acid sequences 125-132 of the D2 domain.
Product Categories/Family for anti-uPAR antibody
References
1. Luther, T et al; Epitope-mapped monoclonal antibodies as tools for functional and morphological analyses of the human urokinase receptor in tumor tissue. Am J Pathol 1997, 150: 1231.
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Product Notes
The uPAR (Catalog #AAA584811) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The uPAR (D2 domain), Human, mAb IIIB11 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's uPAR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB). The typical starting working dilution is 1:10. Researchers should empirically determine the suitability of the uPAR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "uPAR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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