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Western Blot (WB) (B3GNT4 Antaibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)

Rabbit anti-Human, mouse B3GNT4 Polyclonal Antibody | anti-B3GNT4 antibody

B3GNT4 Antibody (Center)

Gene Names
B3GNT4; B3GN-T4; beta3Gn-T4
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
B3GNT4; Polyclonal Antibody; B3GNT4 Antibody (Center); N-acetyllactosaminide beta-1; 3-N-acetylglucosaminyltransferase 4; UDP-GlcNAc:betaGal beta-1; BGnT-4; Beta-1; 3-Gn-T4; Beta3Gn-T4; anti-B3GNT4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This B3GNT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-260 amino acids from the Central region of human B3GNT4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
233-260
Sequence Length
378
Applicable Applications for anti-B3GNT4 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(B3GNT4 Antaibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)

Western Blot (WB) (B3GNT4 Antaibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)

Western Blot (WB)

(B3GNT4 Antaibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)

Western Blot (WB) (B3GNT4 Antaibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the B3GNT4 antibody detected the B3GNT4 protein (arrow).)

Flow Cytometry (FC/FACS)

(B3GNT4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (B3GNT4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-B3GNT4 antibody
This gene encodes a member of the
beta-1,3-N-acetylglucosaminyltransferase protein family. The
encoded enzyme is involved in the biosynthesis of
poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as
a substrate. It is a type II transmembrane protein. [provided by
RefSeq].
References
Shiraishi, N., et al. J. Biol. Chem. 276(5):3498-3507(2001)
Amado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42310
NCBI Official Full Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4
NCBI Official Symbol
B3GNT4
NCBI Official Synonym Symbols
B3GN-T4; beta3Gn-T4
NCBI Protein Information
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4
UniProt Protein Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4
UniProt Gene Name
B3GNT4
UniProt Synonym Gene Names
BGnT-4; Beta-1,3-Gn-T4; Beta-1,3-N-acetylglucosaminyltransferase 4; Beta3Gn-T4
UniProt Entry Name
B3GN4_HUMAN

NCBI Description

This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

B3GNT4: Has a beta-1,3-N-acetylglucosaminyltransferase activity for type 2 oligosaccharides. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.4.1.-; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity; galactosyltransferase activity

Biological Process: keratan sulfate metabolic process; protein amino acid O-linked glycosylation; poly-N-acetyllactosamine biosynthetic process; cellular protein metabolic process; O-glycan processing; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; post-translational protein modification

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Product Notes

The B3GNT4 b3gnt4 (Catalog #AAA9205851) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 233-260. The B3GNT4 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B3GNT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the B3GNT4 b3gnt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GNT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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