Mouse Ubiquitin Conjugating Enzyme E2N Monoclonal Antibody | anti-ube2n antibody
Ubiquitin Conjugating Enzyme E2N (UBE2N, Ubc13, UbcH-ben)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A chromatography
Synonyms
Ubiquitin Conjugating Enzyme E2N; Monoclonal Antibody; Ubiquitin Conjugating Enzyme E2N (UBE2N; Ubc13; UbcH-ben); Anti -Ubiquitin Conjugating Enzyme E2N (UBE2N; anti-ube2n antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6A212
Specificity
Specific for the ~21kD Ubc13 protein. Reactivity has been confirmed with human HepG2 cell lysates, mouse NIH 3T3 cells, and rat Rat2 fibroblasts.
Purity/Purification
Affinity Purified
Purified by Protein A chromatography
Purified by Protein A chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Applicable Applications for anti-ube2n antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot and ELISA.
Dilution: Immunofluorescence: 1-5ug/ml
Western Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml
Dilution: Immunofluorescence: 1-5ug/ml
Western Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml
Immunogen
Recombinant human Ubc13.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ube2n antibody
The Ubc13 protein was first identified for its unique role in ubiquitin chain assembly at the Lys63 residue of ubiquitin, rather than the conventional Lys48 site. Ubc13, which acts as an E2 ubiquitin-conjugating enzyme, must first form a complex with its E2 partner, Mms2, for enzymatic activity. Mms2 belongs to a family of UEV (ubiquitin-conjugating enzyme variant) proteins; Ubc13/UEV complexes are involved in error-free DNA postreplication repair (PRR) in yeast and in the NFkB signaling pathway in humans
Product Categories/Family for anti-ube2n antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-conjugating enzyme E2N
NCBI Official Symbol
ube2n
NCBI Protein Information
ubiquitin-conjugating enzyme E2N; Ubiquitin-conjugating enzyme E2 N
Protein Family
Similar Products
Product Notes
The ube2n (Catalog #AAA607137) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin Conjugating Enzyme E2N (UBE2N, Ubc13, UbcH-ben) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ubiquitin Conjugating Enzyme E2N can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and ELISA. Dilution: Immunofluorescence: 1-5ug/ml Western Blot: 1-3ug/ml ELISA: 0.1-1ug/ml. Researchers should empirically determine the suitability of the ube2n for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin Conjugating Enzyme E2N, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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