Rabbit UBC13 Polyclonal Antibody | anti-UBC13 antibody

UBC13 Antibody

Cat. Num. AAA9410398

• Gene Names: UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Ion exchange chromatography purified
• Synonyms: UBC13; Polyclonal Antibody; UBC13 Antibody; anti-UBC13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Ion exchange chromatography purified
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 152

• Applicable Applications for anti-UBC13 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to 15 amino acids near the C-terminus of human UBC13.
• Target Name: UBC13
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of UBC13 in human small intestine cell lysates with UBC13 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of UBC13 in mouse thymus tissue with UBC13 antibody at 2 ug/mL.)

Related Product Information for anti-UBC13 antibody

Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway. It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway. Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors. Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family. The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation.

Product Categories/Family for anti-UBC13 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 30582585
• NCBI GeneID: 7334
• Molecular Weight: 17,138 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2N
• NCBI Official Symbol: UBE2N
• NCBI Official Synonym Symbols: UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 N
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 N
• UniProt Gene Name: UBE2N
• UniProt Synonym Gene Names: BLU
• UniProt Entry Name: UBE2N_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2N: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family.

Protein type: DNA repair, damage; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; cytosol; nucleolus; nucleoplasm; nucleus; protein complex; UBC13-MMS2 complex; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: activation of NF-kappaB transcription factor; DNA double-strand break processing; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; histone ubiquitination; positive regulation of DNA repair; positive regulation of histone modification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of ubiquitin-protein ligase activity; postreplication repair; protein modification process; protein ubiquitination; proteolysis; regulation of DNA repair; regulation of histone ubiquitination; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway