Rabbit HK1 Polyclonal Antibody | anti-HK1 antibody

HK1 (Hexokinase 1, HK1-ta, HK1-tb, HK1-tc, HKI, HXK1) (PE)

Cat. Num. AAA6420874

• Gene Names: HK1; HKI; HXK1; HK1-ta; HK1-tb; HK1-tc
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: HK1; Polyclonal Antibody; HK1 (Hexokinase 1; HK1-ta; HK1-tb; HK1-tc; HKI; HXK1) (PE); anti-HK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HK1. Species Crossreactivity: mouse and rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HK1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to aa20-300 of human HK1.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using 211280 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using 211280. Blue: DAPI for nuclear staining.)

Testing Data

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Testing Data

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Testing Data

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Related Product Information for anti-HK1 antibody

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

Product Categories/Family for anti-HK1 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 188497754
• NCBI GeneID: 3098
• NCBI Accession #: NP_000179.2
• NCBI GenBank Nucleotide #: NM_000188.2
• UniProt Accession #: P19367; O43443; O43444; O75574; Q5VTC3; Q96HC8; Q9NNZ4; Q9NNZ5; E9PCK0
• Molecular Weight: 102,486 Da
• NCBI Official Full Name: hexokinase-1 isoform HKI
• NCBI Official Synonym Full Names: hexokinase 1
• NCBI Official Symbol: HK1
• NCBI Official Synonym Symbols: HKI; HXK1; HK1-ta; HK1-tb; HK1-tc
• NCBI Protein Information: hexokinase-1; HK I; glycolytic enzyme; hexokinase type I; brain form hexokinase
• UniProt Protein Name: Hexokinase-1
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK1
• UniProt Synonym Gene Names: HK I
• UniProt Entry Name: HXK1_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Uniprot Description

HK1: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Mitochondrial; Carbohydrate Metabolism - galactose; EC 2.7.1.1; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; lipid raft

Molecular Function: hexokinase activity; protein binding; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; carbohydrate metabolic process; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport

Disease: Hemolytic Anemia, Nonspherocytic, Due To Hexokinase Deficiency; Neuropathy, Hereditary Motor And Sensory, Russe Type