Western Blot (WB)
(UBE2H monoclonal antibody, Western Blot analysis of UBE2H expression in HeLa.)
Mouse anti-Human UBE2H Monoclonal Antibody | anti-UBE2H antibody
UBE2H (Ubiquitin-conjugating Enzyme E2 H, UbcH2, Ubiquitin Carrier Protein H, UBCH, Ubiquitin-conjugating Enzyme E2-20K, Ubiquitin-protein Ligase H)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Western Blot (WB)
(UBE2H monoclonal antibody, Western Blot analysis of UBE2H expression in HeLa.)
Western Blot (WB)
(UBE2H monoclonal antibody, Western Blot analysis of UBE2H expression in HeLa.)
Western Blot (WB)
(Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody|Lane 1: UBE2H transfected lysate (20.7kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of UBE2H expression in transfected 293T cell line by UBE2H monoclonal antibody|Lane 1: UBE2H transfected lysate (20.7kD).|Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to UBE2H on HeLa cell. [antibody concentration 10ug/ml])
Testing Data
(Detection limit for recombinant GST tagged UBE2H is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged UBE2H is ~0.03ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
Uniprot Description
UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ligase; Ubiquitin ligase; EC 6.3.2.19; Mitochondrial; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 7q32
Cellular Component: cytoplasm
Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process
Research Articles on UBE2H
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Product Notes
The UBE2H ube2h (Catalog #AAA644243) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The UBE2H (Ubiquitin-conjugating Enzyme E2 H, UbcH2, Ubiquitin Carrier Protein H, UBCH, Ubiquitin-conjugating Enzyme E2-20K, Ubiquitin-protein Ligase H) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2H can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the UBE2H ube2h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL. It is sometimes possible for the material contained within the vial of "UBE2H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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