Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using MBS618654 (upper) or C2088-15 (lower).)
Rabbit anti-Human Caspase 9, Cleavage Site Polyclonal Antibody | anti-casp9 antibody
Caspase 9, Cleavage Site (Asp315) (ICE-LAP6, Mch6)
Gene Names
casp9; casp9-A; caspase9; xCaspase-9
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Caspase 9; Cleavage Site; Polyclonal Antibody; Cleavage Site (Asp315) (ICE-LAP6; Mch6); Anti -Caspase 9; anti-casp9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of the 35kD large subunit of caspase-9 following cleavage at Asp315. Does not react with full length caspase-9 or any other cleaved caspases.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-casp9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunogen
Synthetic peptide (KLH coupled) corresponding to residues around (Asp315) of human caspase-9.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using MBS618654 (upper) or C2088-15 (lower).)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using MBS618654 (upper) or C2088-15 (lower).)
Related Product Information for anti-casp9 antibody
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47kD)/Apaf 1. This complex processes procaspase-9 into a large active subunit (35kD or 17kD) and a small subunit (10kD) by self-cleavage at (Asp315). Cleaved caspase-9 further processes other caspase members, including caspase- 3 and caspase-7, to initiate a caspase cascade, leading to programmed cell death. In addition to self-cleavage, procaspase-9 also can be cleaved in vivo by caspase-3 at Asp330. This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway.
Product Categories/Family for anti-casp9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
caspase 9, apoptosis-related cysteine peptidase
NCBI Official Synonym Full Names
caspase 9, apoptosis-related cysteine peptidase<
NCBI Official Symbol
casp9
NCBI Official Synonym Symbols
casp9-A; caspase9; xCaspase-9
NCBI Protein Information
caspase 9, apoptosis-related cysteine peptidase; caspase-9
Protein Family
Research Articles on casp9
Similar Products
Product Notes
The casp9 (Catalog #AAA618654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase 9, Cleavage Site (Asp315) (ICE-LAP6, Mch6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase 9, Cleavage Site can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100. Researchers should empirically determine the suitability of the casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase 9, Cleavage Site, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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