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Western Blot (WB) (Western Blot analysis of HeLa cells using U2AF1 Monoclonal Antibody at dilution of 1:1000)

Mouse anti-Human U2AF1 Monoclonal Antibody | anti-U2AF1 antibody

U2AF1 Monoclonal Antibody

Gene Names
U2AF1; RN; FP793; U2AF35; U2AFBP; RNU2AF1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein A purification
Synonyms
U2AF1; Monoclonal Antibody; U2AF1 Monoclonal Antibody; DKFZp313J1712; FP793; Human U2 snRNP auxiliary factor small subunit9; RN; RNU2AF1; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor 1; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2AF35; U2AFBP; anti-U2AF1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2c
Clone Number
789
Purity/Purification
Protein A purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Concentration
1mg/mL (varies by lot)
Sequence Length
240
Applicable Applications for anti-U2AF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB 1:500-1:5000
IHC 1:20-1:200
Immunogen
Fusion protein of U2AF35.
Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of HeLa cells using U2AF1 Monoclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of HeLa cells using U2AF1 Monoclonal Antibody at dilution of 1:1000)
Related Product Information for anti-U2AF1 antibody
U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1, synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.
Product Categories/Family for anti-U2AF1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW 28kDa
Observed MW 35-40kDa
NCBI Official Full Name
splicing factor U2AF 35 kDa subunit isoform b
NCBI Official Synonym Full Names
U2 small nuclear RNA auxiliary factor 1
NCBI Official Symbol
U2AF1
NCBI Official Synonym Symbols
RN; FP793; U2AF35; U2AFBP; RNU2AF1
NCBI Protein Information
splicing factor U2AF 35 kDa subunit
UniProt Protein Name
Splicing factor U2AF 35 kDa subunit
Protein Family
UniProt Gene Name
U2AF1
UniProt Synonym Gene Names
U2AF35; U2AFBP

NCBI Description

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

Research Articles on U2AF1

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Product Notes

The U2AF1 u2af1 (Catalog #AAA2542105) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The U2AF1 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's U2AF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB 1:500-1:5000 IHC 1:20-1:200. Researchers should empirically determine the suitability of the U2AF1 u2af1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "U2AF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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