Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using U2AF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit U2AF1 Polyclonal Antibody | anti-U2AF1 antibody
U2AF1 Polyclonal Antibody
Gene Names
U2AF1; RN; FP793; U2AF35; U2AFBP; RNU2AF1
Reactivity
Human, Mouse, Rat, Hamster
Applications
Western Blot, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Purification
Synonyms
U2AF1; Polyclonal Antibody; U2AF1 Polyclonal Antibody; RN; FP793; U2AF35; U2AFBP; RNU2AF1; anti-U2AF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
240
Applicable Applications for anti-U2AF1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:1000
ICC: 1:50 - 1:200
IP: 1:20 - 1:50
ICC: 1:50 - 1:200
IP: 1:20 - 1:50
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human U2AF1
Calculated Molecular Weight
27kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using U2AF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using U2AF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-U2AF1 antibody
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
Product Categories/Family for anti-U2AF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
240
NCBI Official Full Name
Splicing factor U2AF 35 kDa subunit
NCBI Official Synonym Full Names
U2 small nuclear RNA auxiliary factor 1
NCBI Official Symbol
U2AF1
NCBI Official Synonym Symbols
RN; FP793; U2AF35; U2AFBP; RNU2AF1
NCBI Protein Information
splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit
UniProt Protein Name
Splicing factor U2AF 35 kDa subunit
Protein Family
UniProt Gene Name
U2AF1
UniProt Synonym Gene Names
U2AF35; U2AFBP
UniProt Entry Name
U2AF1_HUMAN
Similar Products
Product Notes
The U2AF1 u2af1 (Catalog #AAA129873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The U2AF1 Polyclonal Antibody reacts with Human, Mouse, Rat, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's U2AF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:500 - 1:1000 ICC: 1:50 - 1:200 IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the U2AF1 u2af1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "U2AF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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