Western Blot (WB)
(Western blot detection of Twinfilin-1 in MCF7, C6, 3T3 and Hela cell lysates using Twinfilin-1 mouse mAb (1:5000 diluted).Predicted band size:40KDa.Observed band size:40KDa.)
Mouse Twinfilin-1 Monoclonal Antibody | anti-TWF1 antibody
Anti-Twinfilin-1 Mouse mAb
Western Blot (WB)
(Western blot detection of Twinfilin-1 in MCF7, C6, 3T3 and Hela cell lysates using Twinfilin-1 mouse mAb (1:5000 diluted).Predicted band size:40KDa.Observed band size:40KDa.)
Western Blot (WB)
(Western blot detection of Twinfilin-1 in MCF7, C6, 3T3 and Hela cell lysates using Twinfilin-1 mouse mAb (1:5000 diluted).Predicted band size:40KDa.Observed band size:40KDa.)
Western Blot (WB)
(Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA)
Western Blot (WB)
(Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green). Blue)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green). Blue)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.)
UniPort Summary Function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]
Uniprot Description
A6: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down- regulated. Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: actin cytoskeleton; cell-cell adherens junction; cytoplasm; filopodium; focal adhesion; intercellular junction; myofibril; perinuclear region of cytoplasm
Molecular Function: actin monomer binding; ATP binding; phosphatidylinositol-4,5-bisphosphate binding; protein-tyrosine kinase activity
Biological Process: negative regulation of actin filament polymerization; regulation of actin phosphorylation; sequestering of actin monomers
Research Articles on TWF1
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Product Notes
The TWF1 twf1 (Catalog #AAA476344) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Twinfilin-1 Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Twinfilin-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TWF1 twf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Twinfilin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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