Mouse TulP3 Monoclonal Antibody | anti-TulP3 antibody

TulP3 antibody

Cat. Num. AAA833790

• Gene Names: TULP3; TUBL3
• Applications: Immunofluorescence, Western Blot
• Purity: TulP3 antibody was purified by affinity chromatography.
• Synonyms: TulP3; Monoclonal Antibody; TulP3 antibody; Monoclonal TulP3; Anti-TulP3; Tubby like protein 3; TUBL3; MGC45295; TulP 3; TulP-3; anti-TulP3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6H4
• Purity/Purification: TulP3 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 442

• Applicable Applications for anti-TulP3 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:100; WB: 1:2000
• Biological Significance: TulP3 is a member of the tubby gene family of bipartite transcription factors. Members of this family havebeen identified in plants, vertebrates, and invertebrates, and they share a conserved N-terminal transcriptionactivation region and a conserved C-terminal DNA and phosphatidylinositol-phosphate binding region. The encoded protein binds to phosphoinositides in the plasma membrane via its C-terminal region and probably functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis, for instance, induced by G-protein-coupled-receptor signaling. It plays an important role in neuronal development and function.
• Cross-Reactivity: Human
• Immunogen: TulP3 antibody was raised in mouse using a full length recombinant protein of human TulP3 (NP_003315) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant TulP3 protein using TulP3 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TulP3 protein (Right) or empty vector (Left) detected with TulP3 antibody)

Related Product Information for anti-TulP3 antibody

Mouse monoclonal TulP3 antibody

Product Categories/Family for anti-TulP3 antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21536436
• NCBI GeneID: 7289
• NCBI Accession #: NP_003315
• NCBI GenBank Nucleotide #: NM_003324.4
• UniProt Accession #: O75386; Q8N5B0; B3KNB7; B7Z6A2; D3DUQ4; F8WBZ9
• Molecular Weight: 19,273 Da
• NCBI Official Full Name: tubby-related protein 3 isoform 1
• NCBI Official Synonym Full Names: tubby like protein 3
• NCBI Official Symbol: TULP3
• NCBI Official Synonym Symbols: TUBL3
• NCBI Protein Information: tubby-related protein 3
• UniProt Protein Name: Tubby-related protein 3
• Protein Family: Tubby-related protein
• UniProt Gene Name: TULP3
• UniProt Synonym Gene Names: TUBL3
• UniProt Entry Name: TULP3_HUMAN

NCBI Description

This gene encodes a member of the tubby gene family of bipartite transcription factors. Members of this family have been identified in plants, vertebrates, and invertebrates, and they share a conserved N-terminal transcription activation region and a conserved C-terminal DNA and phosphatidylinositol-phosphate binding region. The encoded protein binds to phosphoinositides in the plasma membrane via its C-terminal region and probably functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis, for instance, induced by G-protein-coupled-receptor signaling. It plays an important role in neuronal development and function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, May 2009]

Uniprot Description

TULP3: Negatively regulates SHH signal transduction. Probably functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to phosphorylated inositide lipids. Belongs to the TUB family.

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular region; nucleolus; plasma membrane; axoneme; nucleus; cilium

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; enzyme binding; protein complex binding; phosphoinositide binding

Biological Process: anterior/posterior pattern formation; G-protein coupled receptor protein signaling pathway; central nervous system neuron differentiation; regulation of transcription, DNA-dependent; negative regulation of smoothened signaling pathway; neural tube closure; embryonic neurocranium morphogenesis; embryonic digit morphogenesis; brain development; embryonic camera-type eye development; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of smoothened signaling pathway in ventral spinal cord patterning