Mouse TrpM7 Monoclonal Antibody | anti-Trpm7 antibody

TrpM7 Antibody: ATTO 390

Cat. Num. AAA804607

• Gene Names: Trpm7; CHAK; CHAK1; Ltpr7; Ltrpc7; LTrpC-7; TRPPLIK; 2310022G15Rik; 4833414K03Rik; 5033407O22Rik
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence
• Synonyms: TrpM7; Monoclonal Antibody; TrpM7 Antibody: ATTO 390; CHAK; CHAK1; LTRPC7; TRP PLIK; Channel kinase 1; anti-Trpm7 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S74-25
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1862

• Applicable Applications for anti-Trpm7 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: ATTO 390
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25. Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Nuclear staining of both neurons and glia.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

Western Blot (WB)

(Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74-25. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-Trpm7 antibody

Background Info: Detects ~220kDa. No cross-reactivity against TrpM6

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (3, 4).

Product Categories/Family for anti-Trpm7 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Brauchi S., Krapivinsky G., Krapivinsky L., Clapham D.E. (2008) Proc Natl Acad Sci USA. 105(24): 8304-8308. 4. Numata T, Okada Y. (2008) J Biol Chem. 283(22): 15097-15103.

NCBI and Uniprot Product Information

• NCBI GI #: 256773287
• NCBI GeneID: 58800
• NCBI Accession #: NP_001157797.1
• NCBI GenBank Nucleotide #: NM_001164325.1
• UniProt Accession #: Q923J1; Q8C7S7; Q8CE54; Q921Y5; Q925B2; Q9CUT2; Q9JLQ1; A2AI58
• Molecular Weight: 212,399 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 7 isoform 2
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 7
• NCBI Official Symbol: Trpm7
• NCBI Official Synonym Symbols: CHAK; CHAK1; Ltpr7; Ltrpc7; LTrpC-7; TRPPLIK; 2310022G15Rik; 4833414K03Rik; 5033407O22Rik
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 7; channel-kinase 1; transient receptor potential M7; long transient receptor potential channel 7; transient receptor potential-related protein, ChaK; transient receptor potential-phospholipase C-interacting kinase
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 7
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: Trpm7
• UniProt Synonym Gene Names: Chak; Ltrpc7; LTrpC-7; LTrpC7; TRP-PLIK
• UniProt Entry Name: TRPM7_MOUSE

Uniprot Description

ChaK1: is an unusual and ubiquitous bifunctional protein containing a transient receptor potential (TRP) ion channel fused to a protein kinase of the Atypical alpha group. Required for early embryonic development and for maintaining cardiac automaticity. The channel conducts Zn(2+), Mg(2+), and Ca(2+) as well as monovalent cations. The kinase can be cleaved from the channel domain. The cleaved kinase translocates to the nucleus where it binds chromatin-remodeling complexes including Polycomb, and phosphorylates specific histone residues. The kinase activity is essential for the channel function, and channel function is critical to maintain physiological intracellular magnesium levels. Plays a key role in oxidative stress-induced cell death. TRP voltage-independent calcium-permeable cation channels are divided into six subfamilies, two of which (TRPC and TRPM) have members that are widely expressed and activated by oxidative stress. Is activated by reactive oxygen/nitrogen species, resulting in cation conductance and anoxic neuronal cell death. This phenotype is rescued by suppression of ChaK1 expression. Related to ChaK2, another Atypical alpha kinase fused to a TRP ion channel protein.

Protein type: Membrane protein, integral; EC 2.7.11.1; Channel, cation; Kinase, protein; Protein kinase, atypical; Membrane protein, multi-pass; ATYPICAL group; Alpha family; ChaK subfamily

Cellular Component: ruffle; neuron projection; membrane; cell soma; synaptic vesicle membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; myosin binding; metal ion binding; calcium channel activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; ion channel activity; cation channel activity; kinase activity; actin binding; ATP binding; protein kinase activity

Biological Process: positive regulation of apoptosis; protein amino acid autophosphorylation; actomyosin structure organization and biogenesis; programmed cell death; protein amino acid phosphorylation; memory; transport; calcium ion transport; calcium-dependent cell-matrix adhesion; ion transport; transmembrane transport; phosphorylation; cation transport