Rabbit anti-Human PCDH20 Polyclonal Antibody | anti-PCDH20 antibody

PCDH20, ID (PCDH20, PCDH13, Protocadherin-20, Protocadherin-13)

Cat. Num. AAA643395

• Gene Names: PCDH20; PCDH13
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PCDH20; Polyclonal Antibody; ID (PCDH20; PCDH13; Protocadherin-20; Protocadherin-13); Anti -PCDH20; anti-PCDH20 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PCDH20 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Immunofluorescence: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-480 amino acids from the Central region of human PCDH20.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCDH20 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCDH20 antibody detected the PCDH20 protein (arrow).)

Immunohistochemistry (IHC)

(PCDH20 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PCDH20 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(PCDH20 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PCDH20 antibody

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain.

Product Categories/Family for anti-PCDH20 antibody

Antibodies; Abs to Cadherin

NCBI and Uniprot Product Information

• NCBI GI #: 190194360
• NCBI GeneID: 64881
• NCBI Accession #: NP_073754.2
• NCBI GenBank Nucleotide #: NM_022843.3
• UniProt Accession #: Q8N6Y1; Q8NDN4; Q9NRT9; A8K1K9; B1AQU2
• Molecular Weight: 104,919 Da
• NCBI Official Full Name: protocadherin-20
• NCBI Official Synonym Full Names: protocadherin 20
• NCBI Official Symbol: PCDH20
• NCBI Official Synonym Symbols: PCDH13
• NCBI Protein Information: protocadherin-20; protocadherin 13; protocadherin-13
• UniProt Protein Name: Protocadherin-20
• Protein Family: Protocadherin
• UniProt Gene Name: PCDH20
• UniProt Synonym Gene Names: PCDH13
• UniProt Entry Name: PCD20_HUMAN

NCBI Description

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDH20: Potential calcium-dependent cell-adhesion protein.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: homophilic cell adhesion