Mouse anti-Human Troponin C Type 1 (TNNC1) Monoclonal Antibody | anti-TNNC1 antibody

Monoclonal Antibody to Troponin C Type 1, Slow (TNNC1)

Cat. Num. AAA2090610

• Gene Names: TNNC1; TNC; TN-C; TNNC; CMD1Z; CMH13
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G affinity chromatography
• Synonyms: Troponin C Type 1 (TNNC1); Monoclonal Antibody; Monoclonal Antibody to Troponin C Type 1; Slow (TNNC1); TNC; TroponinC; Cardiac; Troponin C; slow skeletal and cardiac muscles; anti-TNNC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone Number: C5
• Specificity: The antibody is a mouse monoclonal antibody raised against TNNC1. It has been selected for its ability to recognize TNNC1 in immunohistochemical staining and western blotting.
• Purity/Purification: Protein A + Protein G affinity chromatography
• Form/Format: Liquid
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 161

• Applicable Applications for anti-TNNC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.5-5ug/mL; Immunohistochemistry: 5-30ug/mL; Immunocytochemistry: 5-30ug/mL
• Immunogen: Recombinant Troponin C Type 1, Slow (TNNC1)
• Cross Reactivity: Human, Mouse, Pig
• Buffer Formulation: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Porcine Heart Tissue; Lane3: Porcine Skeletal Muscle Tissue; Lane4: Mouse Heart Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant TNNC1, Human.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507615
• NCBI GeneID: 7134
• NCBI Accession #: NP_003271.1
• NCBI GenBank Nucleotide #: NM_003280.2
• UniProt Accession #: P63316; O14800; P02590; P04463
• Molecular Weight: 18,403 Da
• NCBI Official Full Name: troponin C, slow skeletal and cardiac muscles
• NCBI Official Synonym Full Names: troponin C1, slow skeletal and cardiac type
• NCBI Official Symbol: TNNC1
• NCBI Official Synonym Symbols: TNC; TN-C; TNNC; CMD1Z; CMH13
• NCBI Protein Information: troponin C, slow skeletal and cardiac muscles
• UniProt Protein Name: Troponin C, slow skeletal and cardiac muscles
• Protein Family: Troponin
• UniProt Gene Name: TNNC1
• UniProt Synonym Gene Names: TNNC; TN-C

NCBI Description

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]

Uniprot Description

TNNC1: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13). A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the troponin C family.

Protein type: Calcium-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cardiac Troponin complex; cytosol; troponin complex

Molecular Function: actin filament binding; calcium ion binding; calcium-dependent protein binding; protein binding; protein homodimerization activity; troponin I binding; troponin T binding

Biological Process: cardiac muscle contraction; diaphragm contraction; muscle filament sliding; regulation of ATPase activity; regulation of muscle contraction; regulation of muscle filament sliding speed; response to metal ion; skeletal muscle contraction; transition between fast and slow fiber; ventricular cardiac muscle tissue morphogenesis

Disease: Cardiomyopathy, Dilated, 1z; Cardiomyopathy, Familial Hypertrophic, 13