Western Blot (WB)
(Western Blot detection against Immunogen (70.66kD).)
Mouse anti-Human TRNT1 Monoclonal Antibody | anti-TRNT1 antibody
TRNT1 (CCA tRNA Nucleotidyltransferase 1, Mitochondrial, CCA1, Mitochondrial tRNA Nucleotidyl Transferase, CCA-adding, mt CCA-adding Enzyme, MtCCA, mt tRNA CCA-diphosphorylase, mt tRNA CCA-pyrophosphorylase, mt tRNA Adenylyltransferase, CGI-47)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot detection against Immunogen (70.66kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (70.66kD).)
Western Blot (WB)
(Western Blot analysis of TRNT1 expression in transfected 293T cell line by TRNT1 monoclonal antibody.|Lane 1: TRNT1 transfected lysate (46.4kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of TRNT1 expression in transfected 293T cell line by TRNT1 monoclonal antibody.|Lane 1: TRNT1 transfected lysate (46.4kD).|Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged TRNT1 is 0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged TRNT1 is 0.3ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
The CCA-adding enzyme TRNT1 (EC 2.7.7.25) is an essential enzyme that catalyzes the addition of the CCA terminus to the 3-prime end of tRNA precursors. This reaction is a fundamental prerequisite for mature tRNAs to become aminoacylated and to participate in protein biosynthesis (Lizano et al., 2007 [PubMed 17204286]).[supplied by OMIM, Aug 2009]
Uniprot Description
TRNT1: Isoform 1: Adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA processing; Mitochondrial; Transferase; EC 2.7.7.72
Chromosomal Location of Human Ortholog: 3p25.1
Cellular Component: mitochondrion; intracellular
Molecular Function: tRNA adenylyltransferase activity; ATP binding; tRNA binding
Biological Process: tRNA 3'-terminal CCA addition; tRNA 3'-end processing; protein targeting to mitochondrion
Disease: Sideroblastic Anemia With B-cell Immunodeficiency, Periodic Fevers, And Developmental Delay
Research Articles on TRNT1
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Product Notes
The TRNT1 trnt1 (Catalog #AAA6002869) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRNT1 (CCA tRNA Nucleotidyltransferase 1, Mitochondrial, CCA1, Mitochondrial tRNA Nucleotidyl Transferase, CCA-adding, mt CCA-adding Enzyme, MtCCA, mt tRNA CCA-diphosphorylase, mt tRNA CCA-pyrophosphorylase, mt tRNA Adenylyltransferase, CGI-47) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRNT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the TRNT1 trnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKLQSPEFQS LFTEGLKSLT ELFVKENHEL RIAGGAVRDL LNGVKPQDID FATTATPTQM KEMFQSAGIR MINNRGEKHG TITARLHEEN FEITTLRIDV TTDGRHAEVE FTTDWQKDAE RRDLTINSMF LGFDGTLFDY FNGYEDLKNK KVRFVGHAKQ RIQEDYLRIL RYFRFYGRIV DKPGDHDPET LEAIAENAKG LAGISGERIW VELKKILVGN HVNHLIHLIY DLDVAPYIGL PANASLEEFD KVSKNVDGFS PKPVTLLASL FKVQDDVTKL DLRLKIAKEE KNLGLFIVKN RKDLIKATDS SDPLKPYQDF IIDSREPDAT TRVCELLKYQ GEHCLLKEMQ QWSIPPFPVS GHDIRKVGIS SGKEIGALLQ QLREQWKKSG YQMEKDELLS YIKKT. It is sometimes possible for the material contained within the vial of "TRNT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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