Mouse anti-Human TRMT2A Monoclonal Antibody | anti-TRMT2A antibody

Mouse Monoclonal [clone 1G8] (IgG1) to Human TRMT2A

Cat. Num. AAA249207

• Gene Names: TRMT2A; HTF9C
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A/G Purified
• Synonyms: TRMT2A; Monoclonal Antibody; Mouse Monoclonal [clone 1G8] (IgG1) to Human TRMT2A; Anti-TRMT2A Antibody (clone 1G8) IHC-plus; HTF9C; Human TRMT2A; anti-TRMT2A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1G8
• Specificity: Human TRMT2A
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 625

• Applicable Applications for anti-TRMT2A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:2000)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human TRMT2A(NP_073564) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: TRMT2A antibody was raised against full length human recombinant protein of human TRMT2A(NP_073564) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TRMT2A antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT2A.)

NCBI and Uniprot Product Information

• NCBI GI #: 51173878
• NCBI GeneID: 27037
• NCBI Accession #: NP_073564.3
• NCBI GenBank Nucleotide #: NM_022727.5
• UniProt Accession #: Q8IZ69; Q32P57; Q96ME6; Q9H732; D3DX25
• Molecular Weight: 61,979 Da
• NCBI Official Full Name: tRNA (uracil-5-)-methyltransferase homolog A isoform a
• NCBI Official Synonym Full Names: tRNA methyltransferase 2 homolog A (S. cerevisiae)
• NCBI Official Symbol: TRMT2A
• NCBI Official Synonym Symbols: HTF9C
• NCBI Protein Information: tRNA (uracil-5-)-methyltransferase homolog A; tRNA (uracil-5-)-methyltransferase homolog A; hpaII tiny fragments locus 9c protein; TRM2 tRNA methyltransferase 2 homolog A
• UniProt Protein Name: tRNA (uracil-5-)-methyltransferase homolog A
• Protein Family: tRNA
• UniProt Gene Name: TRMT2A
• UniProt Synonym Gene Names: HTF9C
• UniProt Entry Name: TRM2A_HUMAN

NCBI Description

The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

HTF9C: May be involved in nucleic acid metabolism and/or modifications. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 22q11.21

Molecular Function: nucleotide binding; RNA methyltransferase activity

Biological Process: RNA processing; RNA methylation