Rat anti-Mouse TREM-2b Monoclonal Antibody | anti-Trem2 antibody

TREM-2b (Trem2b, Triggering Receptor Expressed on Monocytes 2) (PE)

Cat. Num. AAA6012484

• Gene Names: Trem2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Mouse
• Applications: Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography prior to conjugation.
• Synonyms: TREM-2b; Monoclonal Antibody; TREM-2b (Trem2b; Triggering Receptor Expressed on Monocytes 2) (PE); Anti -TREM-2b (Trem2b; anti-Trem2 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 7H10
• Specificity: Recognizes mouse TREM-2b.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography prior to conjugation.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with PE.

• Applicable Applications for anti-Trem2 antibody: Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry and Immunocytochemistry.; Dilution: Flow Cytometry: Neat. Use 10ul to label 1x10e5 cells.
• Immunogen: NS0-derived recombinant mouse TREM-2b extracellular domain
• Preparation and Storage: May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Related Product Information for anti-Trem2 antibody

TREM-2 is an Ig superfamily cell surface receptor that activates a number of myeloid cell types. It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC. A single human TREM-2 gene has been described, however, two closely related orthologs were reported in mouse. The proteins differ by only three amino acids and were designated TREM-2a and TREM-2b. TREM-2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail. It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM-2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals. Ligation of TREM-2 on immature DC with anti-TREM-2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM-2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM-2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts. In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM-2 deficient individuals.

Product Categories/Family for anti-Trem2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 440918700
• NCBI GeneID: 83433
• NCBI Accession #: NP_001259007.1
• NCBI GenBank Nucleotide #: NM_001272078.1
• UniProt Accession #: Q99NH8; Q8CGK4; Q8CIA6; Q99NH9; Q9JL34
• Molecular Weight: 24,527 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 2
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: Trem2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: Trem2
• UniProt Synonym Gene Names: Trem2a; Trem2b; Trem2c; TREM-2
• UniProt Entry Name: TREM2_MOUSE

NCBI Description

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; intracellular membrane-bound organelle; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; peptidoglycan binding; transmembrane receptor activity; lipopolysaccharide binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; lipopolysaccharide-mediated signaling pathway; innate immune response; positive regulation of calcium-mediated signaling; signal transduction; positive regulation of antigen processing and presentation of peptide antigen via MHC class II