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Western Blot (WB) (Western Blot: Sample: Recombinant TMEM27, Human.)

Mouse anti-Human Transmembrane Protein 27 Monoclonal Antibody | anti-TMEM27 antibody

Transmembrane Protein 27 (TMEM27) Monoclonal Antibody

Gene Names
CLTRN; NX17; NX-17; TMEM27
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Transmembrane Protein 27; Monoclonal Antibody; Transmembrane Protein 27 (TMEM27) Monoclonal Antibody; NX17; Collectrin; anti-TMEM27 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C5
Specificity
The antibody is a mouse monoclonal antibody raised against TMEM27. It has been selected for its ability to recognize TMEM27 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Length
222
Applicable Applications for anti-TMEM27 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:100-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2082305
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant TMEM27, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant TMEM27, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Glioma Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Glioma Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,235 Da
NCBI Official Full Name
collectrin
NCBI Official Synonym Full Names
collectrin, amino acid transport regulator
NCBI Official Symbol
CLTRN
NCBI Official Synonym Symbols
NX17; NX-17; TMEM27
NCBI Protein Information
collectrin
UniProt Protein Name
Collectrin
Protein Family
UniProt Gene Name
TMEM27

NCBI Description

This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]

Uniprot Description

TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: brush border membrane; cytoplasm; integral component of membrane

Molecular Function: metallopeptidase activity; peptidyl-dipeptidase activity; protein binding

Biological Process: positive regulation of amino acid transport; positive regulation of calcium ion-dependent exocytosis; proteolysis

Research Articles on TMEM27

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Product Notes

The TMEM27 tmem27 (Catalog #AAA2032075) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Transmembrane Protein 27 (TMEM27) Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Transmembrane Protein 27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:100-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TMEM27 tmem27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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