Testing Data
(Detection of TCERG1 using MBS602606. Samples: Whole cell lysate from human A2058 (H, 50ug) , mouse NIH3T3 (M, 50ug) and rat F2408 (R, 50ug) cells. Predicted molecular weight: 123kD)
Mouse Transcription Elongation Regulator 1 Monoclonal Antibody | anti-TCERG1 antibody
Transcription Elongation Regulator 1 (TCERG1, CA150, MGC133200, TAF2S, TATA box-binding protein-associated factor 2S, Transcription factor CA150, Urn1)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Immunofluorescence (IC): 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with
0.1% Triton X-100 in PBS.
Western Blot: 0.2-2ug/ml
Flow cytometry: 0.5-2ug/ml. HeLa cells with 2% paraformaldehyde/PBS and then permeabilized in 90% methanol.
Testing Data
(Detection of TCERG1 using MBS602606. Samples: Whole cell lysate from human A2058 (H, 50ug) , mouse NIH3T3 (M, 50ug) and rat F2408 (R, 50ug) cells. Predicted molecular weight: 123kD)
Testing Data
(Detection of TCERG1 using MBS602606. Samples: Whole cell lysate from human A2058 (H, 50ug) , mouse NIH3T3 (M, 50ug) and rat F2408 (R, 50ug) cells. Predicted molecular weight: 123kD)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with O8056-05A (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with O8056-05A (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS602606.)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS602606.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [provided by RefSeq]
Uniprot Description
TCERG1: Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; RNA-binding; Spliceosome
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleolus; nucleus; actin cytoskeleton
Molecular Function: protein binding; transcription coactivator activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter
Research Articles on TCERG1
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Product Notes
The TCERG1 tcerg1 (Catalog #AAA602606) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Transcription Elongation Regulator 1 (TCERG1, CA150, MGC133200, TAF2S, TATA box-binding protein-associated factor 2S, Transcription factor CA150, Urn1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Transcription Elongation Regulator 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence (IC) and Western Blot. Dilution: Immunofluorescence (IC): 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Western Blot: 0.2-2ug/ml Flow cytometry: 0.5-2ug/ml. HeLa cells with 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Researchers should empirically determine the suitability of the TCERG1 tcerg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transcription Elongation Regulator 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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