Mouse anti-Human TRAF3IP2 / ACT1 Monoclonal Antibody | anti-TRAF3IP2 antibody

Mouse Monoclonal [clone 4A3] (IgG2a,k) to Human TRAF3IP2 / ACT1

Cat. Num. AAA246274

• Gene Names: TRAF3IP2; ACT1; CIKS; C6orf2; C6orf4; C6orf5; C6orf6; CANDF8; PSORS13
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: TRAF3IP2 / ACT1; Monoclonal Antibody; Mouse Monoclonal [clone 4A3] (IgG2a; k) to Human TRAF3IP2 / ACT1; Anti-TRAF3IP2 / ACT1 Antibody (clone 4A3) IHC-plus; TRAF3IP2; C6orf2; C6orf4; C6orf5; CIKS; DKFZP586G0522; Connection to IKK and SAPK/JNK; ACT1; Adapter protein CIKS; NFkB-activating protein ACT1; PSORS13; TRAF3-interacting protein 2; C6orf6; TRAF3 interacting protein 2; Human TRAF3IP2; anti-TRAF3IP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4A3
• Specificity: Human CIKS
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 564

• Applicable Applications for anti-TRAF3IP2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: TRAF3IP2 (AAH02823, 451 a.a. ~ 566 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: TRAF3IP2 / ACT1 antibody was raised against tRAF3IP2 (AAH02823, 451 a.a. ~ 566 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TRAF3IP2 / ACT1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to TRAF3IP2 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of TRAF3IP2 expression in HeLa nuclear extracts with TRAF3IP2 monoclonal antibody, clone 4A3.)

ELISA (EIA)

(Detection limit for recombinant GST tagged TRAF3IP2 is approximately 1 ng/ml as a capture antibody.)

Related Product Information for anti-TRAF3IP2 antibody

Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB associates with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase (IKK) complex that contains IKKa, IKKb, and IKKg.

NCBI and Uniprot Product Information

• NCBI GI #: 22538426
• NCBI GeneID: 10758
• NCBI Accession #: NP_671733.1
• NCBI GenBank Nucleotide #: NM_147200.1
• UniProt Accession #: O43734; Q5R3A3; Q7Z6Q1; Q7Z6Q2; Q7Z6Q3; Q9H5W2; Q9H6Y3; Q9NS14; Q9UG72; B2RAY9; E1P555
• Molecular Weight: 63,457 Da
• NCBI Official Full Name: TRAF3 interacting protein 2 isoform 1
• NCBI Official Synonym Full Names: TRAF3 interacting protein 2
• NCBI Official Symbol: TRAF3IP2
• NCBI Official Synonym Symbols: ACT1; CIKS; C6orf2; C6orf4; C6orf5; C6orf6; CANDF8; PSORS13
• NCBI Protein Information: adapter protein CIKS
• UniProt Protein Name: Adapter protein CIKS
• Protein Family: Adapter protein
• UniProt Gene Name: TRAF3IP2
• UniProt Synonym Gene Names: C6orf2; C6orf4; C6orf5; C6orf6; ACT1
• UniProt Entry Name: CIKS_HUMAN

NCBI Description

This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Several alternative transcripts encoding different isoforms have been identified. Another transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

TRAF3IP2: Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK. Defects in TRAF3IP2 are the cause of susceptibility to psoriasis type 13 (PSORS13). PSORS13 is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Molecular Function: protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; immunoglobulin secretion; B cell apoptosis; humoral immune response

Disease: Candidiasis, Familial, 8; Psoriasis 13, Susceptibility To