Mouse anti-Human Toll-like Receptor 2 (TLR2) Monoclonal Antibody

Mouse Anti-Human TLR2-BIOT

Cat. Num. AAA670138

• Reactivity: Human
• Applications: Flow Cytometry
• Synonyms: Toll-like Receptor 2 (TLR2); Monoclonal Antibody; Mouse Anti-Human TLR2-BIOT; Mouse Anti-Human TLR2 - Biotin; Mouse Anti-Human Toll-like Receptor 2 (TLR2); anti-Toll-like Receptor 2 (TLR2) antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 2B4A1
• Specificity: Human Toll-like Receptor 2 (TLR2); The Toll-like receptor (TLR) family is comprised of Type I transmembrane proteins characterized by an extracellular leucine-rich domain and a cytoplasmic tail that contains a conserved region call the Toll/IL-1 (TIR) domain. First discovered in Drosophila, TLRs recognize specific molecular patterns that are present in microbial components and respond to these components in order eliminate or limit invading microbes. To date, ten mammalian homologs of TLRs have been described. Activation of TLR2 leads to the initiation of apoptosis and the activation of the NK- kappa B pathway by the adaptor molecule MyD88. TLR2 recognizes and is activated by components from a broad variety of microorganisms including lipoproteins from Gram-negative bacteria, Mycoplasma, spirochetes, peptidoglycan and LTAs from Gram-positive bacteria, lipoarabinomannan from mycobacteria, glycoinositolphospholipids, phenolsoluble modulin, zymosan from yeast and neisserial porins. TLR2 has been reported to functionally associate with TLR1 and TLR6 in order to recognize various lipopeptides.1-16
• Form/Format: Biotin (BIOT) Conjugate
• Sequence Length: 123

• Applicable Applications for anti-Toll-like Receptor 2 (TLR2) antibody: Flow Cytometry
• Application Notes: Flow Cytometry Suggested Dilution: 1ug/10^6
• Characterization: To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or flow cytometry to conform with the characteristics of a standard reference reagent.
• Preparation and Storage: The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.1 mg in 0.2 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS. Aliquot and store at or below -20 degree C. ; With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

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2. Schwandner, R., R. Dziarski, H. Wesche, M. Rothe, and C.J. Kirschning. 1999. J. Biol. Chem. 274:17406.
3. Campos, M.A., I.C. Almeida, O. Takeuchi, S. Akira, E.P. Valente, D.O. Procópio, L.R. Travassos, J.A. Smith, D.T. Golenbock, and R.T. Gazzinelli. 2001. J. Immunol. 167:416.
4. Aliprantis, A.O., R. Yang, D.S. Weiss, P. Godowski, and A. Zychlinsky. 2000. EMBO J. 19:3325.
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8. Aliprantis, A.O., R. Yang, M. R. Mark, S. Suggett, B. Devaux, J.D. Radolf, G.R. Klimpel, P. Godowski, and A. Zychlinsky. 1999. Science 285:736.
9. Yoshimura, A., E. Lien, R.R. Ingalls, E. Tuomanen, R. Dziarski, and D. Golenbock. 1999. J. Immunol. 163:1.
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12. Massari, P., P. Henneke, Y. Ho, E. Latz, D.T. Golenbock, and L.M. Wetzler. 2002. J. Immunol. 168:1533.
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NCBI and Uniprot Product Information

• NCBI GI #: 242254876
• NCBI Official Full Name: toll-like receptor 2

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]