Human Ghrelin O-acyltransferase ELISA Kit | MBOAT4 elisa kit

Human Ghrelin O-acyltransferase ELISA Kit

Cat. Num. AAA765534

• Gene Names: MBOAT4; GOAT; OACT4; FKSG89
• Reactivity: Human
• Synonyms: Ghrelin O-acyltransferase; Human Ghrelin O-acyltransferase ELISA Kit; MBOAT4/OACT4/EC 2.3.1.-/ghrelin O-acyltransferase/membrane bound O-acyltransferase domain containing 4/Membrane-bound O-acyltransferase domain-containing protein 4/OACT4/O-acyltransferase (membrane bound) domain containing 4/O-acyltransferase domain-containing protein 4; MBOAT4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MBOAT4 . No significant cross-reactivity or interference between MBOAT4 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between MBOAT4 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 435

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MBOAT4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MBOAT4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MBOAT4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MBOAT4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MBOAT4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MBOAT4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MBOAT4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 189571629
• NCBI GeneID: 619373
• NCBI Accession #: NP_001094386.1
• NCBI GenBank Nucleotide #: NM_001100916.1
• Molecular Weight: 37,721 Da
• NCBI Official Full Name: ghrelin O-acyltransferase
• NCBI Official Synonym Full Names: membrane bound O-acyltransferase domain containing 4
• NCBI Official Symbol: MBOAT4
• NCBI Official Synonym Symbols: GOAT; OACT4; FKSG89
• NCBI Protein Information: ghrelin O-acyltransferase
• UniProt Protein Name: Ghrelin O-acyltransferase
• Protein Family: Ghrelin O-acyltransferase
• UniProt Gene Name: MBOAT4
• UniProt Synonym Gene Names: GOAT; OACT4; O-acyltransferase domain-containing protein 4
• UniProt Entry Name: MBOA4_HUMAN

Uniprot Description

MBOAT4: Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Belongs to the membrane-bound acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.-; Membrane protein, multi-pass; Membrane protein, integral; Transferase; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Molecular Function: serine O-acyltransferase activity

Biological Process: cellular protein metabolic process; peptidyl-serine octanoylation