Rat anti-Mouse TNF-RII Monoclonal Antibody | anti-TNF-RII antibody

TNF-RII, Mouse, mAb FITC

Cat. Num. AAA584057

• Gene Names: TNFRSF1B; TNF-RII/TNFRSF1B
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: TNF-RII; Monoclonal Antibody; Mouse; mAb FITC; Tumor necrosis factor receptor superfamily member 1B; CD120b; TNF-R2; p75; p80 TNF-alpha receptor; anti-TNF-RII antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 1% bovine serum albumin.
• Sequence Length: 31

• Applicable Applications for anti-TNF-RII antibody: F, Flow Cytometry (FC/FACS), FS, Immuno Assay (IA), Immunoprecipitaion (IP), Western Blot (WB)
• Application Notes: Application Notes: FS: In most cases 2 ug/ml acts agonistically; Application Use: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Conjugate: FITC
• Positive Control: RAW264.7 cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-TNF-RII antibody

Product Description: The monoclonal antibody HM102 recognizes the extracellular part of membrane-bound TNF-RII as well as the soluble form of TNF-RII which is generated by proteolytic cleavage of the extracellular domain. The soluble form can still bind TNF-alpha with high affinity and functions as a TNF-alpha antagonist. TNF-alpha is an important signalling protein in the immune system which can activate inflammatory responses, induce apoptosis, regulate cellular proliferation, and may even promote cancer progression. TNF-alpha can bind to two structurally distinct membrane receptors, TNF-RI and TNFRII, which have both distinct and overlapping downstream signaling cascades. TNFRI is believed to be expressed on nearly all cell types, whereas TNFRII exhibits more restricted expression, being found on certain subpopulations of immune cells and several other cell types. A dominant role of TNFRII has been shown in thymocyte activation by TNF-alpha, whereas induction of cytotoxicity and other functions are mediated largely by TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is activated only by the membrane bound form of TNF-alpha. The antibody is a agonistic receptor modulating antibody. It enhances in vitro TNF alpha responses by increasing the affinity of the soluble form of TNF-alpha for TNF-RII.

References

1. Tacchini-Cottier, F et al; Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice. J Immunol 1998, 160: 6182 2. Brekke, O et al. Specificity of endogenous fatty acid release during tumor necrosis factor-induced apoptosis in WEHI164 fibrosarcoma cells. J of Lipid res. 1999, 40:2223
3. Marchetti, L et al; Tumor Necrosis Factor (TNF)-mediated neuroprotection against glutamate-induced excitotoxicity is enhanced by N-methyl-D-aspartate receptor activation. J Biol Chem 2004, 279: 32869
4. Taoufik, E et al; FLIPL protects neurons against in vivo ischemia and in vitro glucose deprivation-induced cell death. J Neurosci 2007, 27: 6633
5. Veroni, C et al; Activation of TNF receptor 2 in microglia promotes induction of anti-inflammatory pathways. Mol Cell Neurosci 2010, 45: 234
6. Chee, J et al. TNF receptor 1 deficiency increases regulatory T cell function in nonobese diabetic mice. J immunol 2011, 187:1702-1712

NCBI and Uniprot Product Information

• NCBI GI #: 315423985
• NCBI GeneID: 338033
• Molecular Weight: 3,217 Da
• NCBI Official Full Name: tumor necrosis factor receptor type two, partial
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1B
• NCBI Official Symbol: TNFRSF1B
• NCBI Official Synonym Symbols: TNF-RII/TNFRSF1B
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1B
• UniProt Protein Name: Tumor necrosis factor receptor type two
• UniProt Gene Name: TNF-RII/TNFRSF1B
• UniProt Entry Name: E7CZZ7_BOVIN