Mouse CTH Monoclonal Antibody | anti-CTH antibody

CTH (Cystathionase (Cystathionine gamma-Lyase), MGC9471) (HRP)

Cat. Num. AAA6181385

• Applications: Western Blot
• Purity: Purified
• Synonyms: CTH; Monoclonal Antibody; CTH (Cystathionase (Cystathionine gamma-Lyase); MGC9471) (HRP); Cystathionase (Cystathionine gamma-Lyase); MGC9471; anti-CTH antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2E12-1C10
• Specificity: Recognizes CTH.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 405

• Applicable Applications for anti-CTH antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: CTH (AAH15807, 1aa-405aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CTH monoclonal antibody (M02), clone 2E12-1C10 Western Blot analysis of CTH expression in K-562 (Cat # L009V1).)

Testing Data

(Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.)

Product Categories/Family for anti-CTH antibody

Antibodies; Enzymes

References

1. The integrated stress response modulates cellular redox state via induction of cystathionine ?^-lyase: cross-talk between the integrated stress response and thiol metabolism.Dickhout JG, Carlisle RE, Jerome DE, Mohammed-Ali Z, Jiang H, Yang G, Mani S, Garg SK, Banerjee R, Kaufman RJ, Maclean KN, Wang R, Austin RC.J Biol Chem. 2012 Jan 3. 2. A novel transgenic mouse model of CBS deficient homocystinuria does not incur hepatic steatosis or fibrosis and exhibits a hypercoagulative phenotype that is ameliorated by betaine treatment.Maclean KN, Sikora J, Kozich V, Jiang H, Greiner LS, Kraus E, Krijt J, Overdier KH, Collard R, Brodsky GL, Meltesen L, Crnic LS, Allen RH, Stabler SP, Elleder M, Rozen R, Patterson D, Kraus JP.Mol Genet Metab. 2010 Jun 23. 3.Hydrogen sulfide as a mediator of human corpus cavernosum smooth-muscle relaxation. dEmmanuele di Villa Bianca R, Sorrentino R, Maffia P, Mirone V, Imbimbo C, Fusco F, De Palma R, Ignarro LJ, Cirino G.Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4513-8. Epub 2009 Mar 2.

NCBI and Uniprot Product Information

• NCBI GI #: 16041833
• NCBI GeneID: 1491
• NCBI Official Full Name: Cystathionase (cystathionine gamma-lyase)
• NCBI Official Synonym Full Names: cystathionine gamma-lyase
• NCBI Official Symbol: CTH
• NCBI Protein Information: cystathionine gamma-lyase
• Protein Family: Cystathionine gamma-lyase

NCBI Description

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]