Rabbit anti-Human MAGEB2 Polyclonal Antibody | anti-MAGEB2 antibody

MAGEB2, NT (Melanoma-associated Antigen B2, MAGE-B2 Antigen, DSS-AHC Critical Interval MAGE Superfamily 6, DAM6, MAGE XP-2 Antigen, Cancer/Testis Antigen 3.2, CT3.2) (Azide free) (HRP)

Cat. Num. AAA6486323

• Gene Names: MAGEB2; DAM6; CT3.2; MAGE-XP-2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAGEB2; Polyclonal Antibody; NT (Melanoma-associated Antigen B2; MAGE-B2 Antigen; DSS-AHC Critical Interval MAGE Superfamily 6; DAM6; MAGE XP-2 Antigen; Cancer/Testis Antigen 3.2; CT3.2) (Azide free) (HRP); anti-MAGEB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAGEB2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MAGEB2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MAGEB2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAGEB2(arrow) using rabbit polyclonal MAGEB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAGEB2 gene (Lane 2)(Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MAGEB2 antibody

Antibodies; Cancer Markers

References

1. Park, J.H., et al., Mol. Cells 13(2):288-295 (2002). 2. Lurquin, C., et al., Genomics 46(3):397-408 (1997). 3. Dabovic, B., et al., Mamm. Genome 6(9):571-580 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 222418639
• NCBI GeneID: 4113
• NCBI Accession #: NP_002355
• NCBI GenBank Nucleotide #: NM_002364
• UniProt Accession #: O15479
• Molecular Weight: 35kDa
• NCBI Official Full Name: melanoma-associated antigen B2
• NCBI Official Synonym Full Names: MAGE family member B2
• NCBI Official Symbol: MAGEB2
• NCBI Official Synonym Symbols: DAM6; CT3.2; MAGE-XP-2
• NCBI Protein Information: melanoma-associated antigen B2
• UniProt Protein Name: Melanoma-associated antigen B2
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEB2
• UniProt Synonym Gene Names: CT3.2; DAM6
• UniProt Entry Name: MAGB2_HUMAN

NCBI Description

This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region. It is expressed in testis and placenta, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGE-B2: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xp21.3