Mouse anti-Human TNF-alpha APC1, Cachectin, Differentiation inducing factor Monoclonal Antibody | anti-TNFA antibody

TNF-alpha APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand Superfamily member

Cat. Num. AAA6236565

• Reactivity: Human
• Applications: FLISA
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: TNF-alpha APC1; Cachectin; Differentiation inducing factor; Monoclonal Antibody; Differentiation inducing factor (DIF); Macrophage cytotoxic factor (MCF); Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand Superfamily member; APC1; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; anti-TNFA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: TNF655
• Specificity: Recognizes human TNF-alpha.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-TNFA antibody: FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Recombinant human TNF-alpha. Cellular Localization: Cytoplasmic and extracellular (secreted)
• Conjugate: MaxLight750
• Positive Control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-TNFA antibody

This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Product Categories/Family for anti-TNFA antibody

Antibodies; Growth Factors; Cytokines

References

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