Rat anti-Mouse CD274/PD-L1 Monoclonal Antibody | anti-CD274/PD-L1 antibody

Anti-Mouse CD274/PD-L1 Monoclonal Antibody (PE Conjugated) [10F.9G2]

Cat. Num. AAA2558961

• Gene Names: Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD274/PD-L1; Monoclonal Antibody; Anti-Mouse CD274/PD-L1 Monoclonal Antibody (PE Conjugated) [10F.9G2]; B7-H1; PD-L1; Programmed cell death ligand 1; B7 homolog 1; B7-H; B7H1; PDL1; PDCD1L1; PDCD1LG1; anti-CD274/PD-L1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: 10F.9G2
• Form/Format: PBS with 0.05% Proclin300, 1% BSA
• Concentration: 0.5 mg/mL (varies by lot)
• Sequence Length: 290

• Applicable Applications for anti-CD274/PD-L1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is <= 0.5 ug per 10⁶ cells in 100 ul volume or 100 ul of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
• Conjugation: PE
• Preparation and Storage: Store at 2-8 degree C and protect from prolonged exposure to light. Do not freeze.

Flow Cytometry (FC/FACS)

(C57BL/6 murine splenocytes are stained with Anti-Mouse PD-L1 Monoclonal Antibody(PE Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.)

Related Product Information for anti-CD274/PD-L1 antibody

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-gamma activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-gamma, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

NCBI and Uniprot Product Information

• NCBI GI #: 11230798
• NCBI GeneID: 60533
• NCBI Accession #: NP_068693.1
• NCBI GenBank Nucleotide #: NP_068693.1
• UniProt Accession #: Q9EP73
• NCBI Official Full Name: programmed cell death 1 ligand 1
• NCBI Official Synonym Full Names: CD274 antigen
• NCBI Official Symbol: Cd274
• NCBI Official Synonym Symbols: B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• NCBI Protein Information: programmed cell death 1 ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: Cd274
• UniProt Synonym Gene Names: B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_MOUSE

NCBI Description

The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis. [provided by RefSeq, Sep 2015]

Uniprot Description

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of T cell proliferation; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction; positive regulation of cell migration