Mouse TNF alpha Monoclonal Antibody | anti-TNF antibody

TNF alpha (Biotin)

Cat. Num. AAA6249372

• Gene Names: TNF; TNF-alpha
• Reactivity: Bovine, Sheep
• Applications: ELISA, Western Blot, Flow Cytometry, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: TNF alpha; Monoclonal Antibody; TNF alpha (Biotin); anti-TNF antibody

• Host: Mouse
• Reactivity: Bovine, Sheep
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: VPM61
• Specificity: Recognizes sheep TNF alpha. Species Crossreactivity: bovine.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TNF antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: Frozen sections.; Optimal dilutions to be determined by the researcher. Other applications not tested.
• Immunogen: Recombinant protein corresponding to sheep TNF alpha.
• Conjugate: Biotin
• Preparation and Storage: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at-20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.; Note: Applications are based on unconjugated antibody.

Testing Data

(Intracellular staining of transfected CHO cells using MBS6249372 (1:200) with a secondary IgG (PE) rabbit anti-mouse)

Related Product Information for anti-TNF antibody

Recognizes sheep tumor necrosis factor (TNF) alpha, a 17.5kD cytokine expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.

The production of TNF alpha is induced by a variety of factors,dependant upon cell type and includes bacterial toxins, IL-1,PDGF, IFN beta, NGF, oncostatin M and viral infections. Thepresence of TNF alpha is responsible for diverseimmunomodulatory, anti-tumor and toxic effects and undercertain conditions is also capable of self-stimulation andinhibition.

Product Categories/Family for anti-TNF antibody

Antibodies; Growth Factors; Cytokines

References

1. Vilmos, P. et al. (1996) Phylogenetically concerved epitopes of leukocyte antigens. Vet. Immunology and Immunopathology 52:415-426. 2. Green, I.R. et al. (1993) Expression and characterization of bioactive recombinant ovine TNF-alpha: some species specificity in cytotoxic response to TNF. Cytokine 5:213-223.

NCBI and Uniprot Product Information

• NCBI GI #: 67845989
• NCBI GeneID: 443540
• NCBI Accession #: NP_001020031.1
• NCBI GenBank Nucleotide #: NM_001024860.1
• UniProt Accession #: P23383
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: TNF-alpha
• NCBI Protein Information: tumor necrosis factor; TNF-a; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
• UniProt Protein Name: Tumor necrosis factor
• Protein Family: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_SHEEP

Uniprot Description

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.