Placental Lactogen Recombinant Protein | PL recombinant protein

Recombinant Placental Lactogen (PL)

Cat. Num. AAA2028910

• Gene Names: CSH1; PL; CSA; CS-1; CSMT; hCS-1; hCS-A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Placental Lactogen; Recombinant Placental Lactogen (PL); PL recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 5.9 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QTV PLSRLFDHAM LQAHRAHQLA IDTYQEFEET YIPKDQKYSF LHDSQTSFCF SDSIPTPSNM EETQQKSNLE LLRISLLLIE SWLEPVRFLR SMFANNLVYD TSDSDDYHLL KDLEEGIQTL MGRLEDGSRRTGQILKQTYS KFDTNSHNHD ALLKNYGLLY CF
• Sequence Length: 217

• Applicable Applications for PL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 23.1kDa
• Accurate Molecular Mass (KD): 25kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Gln28~Phe192
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 190034
• NCBI GeneID: 1442
• Molecular Weight: 25,020 Da
• NCBI Official Full Name: placental lactogen
• NCBI Official Synonym Full Names: chorionic somatomammotropin hormone 1 (placental lactogen)
• NCBI Official Symbol: CSH1
• NCBI Official Synonym Symbols: PL; CSA; CS-1; CSMT; hCS-1; hCS-A
• NCBI Protein Information: chorionic somatomammotropin hormone 1
• UniProt Protein Name: Chorionic somatomammotropin hormone 1
• UniProt Gene Name: CSH1
• UniProt Synonym Gene Names: Choriomammotropin; PL
• UniProt Entry Name: CSH1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CSH1: Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization. Belongs to the somatotropin/prolactin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular region

Molecular Function: hormone activity; metal ion binding