Mouse TLR9 Monoclonal Antibody | anti-TLR9 antibody

TLR9, Mouse, mAb 5G5, FITC

Cat. Num. AAA584433

• Gene Names: TLR9; CD289
• Reactivity: Reactivity: Mouse. Cross reactivity: Canine, Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: TLR9; Monoclonal Antibody; Mouse; mAb 5G5; FITC; anti-TLR9 antibody

• Host: Mouse
• Reactivity: Reactivity: Mouse. Cross reactivity: Canine, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1.0% bovine serum albumin and 0.02% sodium azide.
• Sequence Length: 1032

• Applicable Applications for anti-TLR9 antibody: F, Flow Cytometry (FC/FACS), Immunoassay (IA), P, Western Blot (WB)
• Application Notes: Application Notes: The monoclonal antibody 5G5 can be used for flow cytometry, Western blotting and immuno assays as detection antibody. Furthermore, the monoclonal antibody 5G5 is useful for immunohistology on frozen and paraffin sections. The monoclonal antibody 5G5 stains RAW macrophages and TLR9 (CD289) transfected HEK293 cells. The antibody is weakly cross reactive with human TLR9.; Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Conjugate: FITC
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TLR9 antibody

Product Description: The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9.

References

1. Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958 2. Rutz, M et al; Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner. Eur J Immunol 2004, 34: 2541
3. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184

NCBI and Uniprot Product Information

• NCBI GI #: 74054321
• NCBI GeneID: 54106
• Molecular Weight: 114,237 Da
• NCBI Official Full Name: TLR9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Official Synonym Symbols: CD289
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR9
• UniProt Synonym Gene Names: UNQ5798/PRO19605
• UniProt Entry Name: TLR9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; lysosome; basolateral plasma membrane; endoplasmic reticulum; apical plasma membrane; cytoplasm; plasma membrane; integral to membrane; extracellular region; endosome membrane; endosome

Molecular Function: interleukin-1 receptor binding; siRNA binding; transmembrane receptor activity

Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; defense response to Gram-negative bacterium; positive regulation of interleukin-18 production; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; negative regulation of interleukin-8 production; positive regulation of interferon-beta production; negative regulation of interleukin-6 production; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; positive regulation of interferon-beta biosynthetic process; defense response to bacterium; insulin receptor signaling pathway; toll-like receptor signaling pathway; negative regulation of toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response