Flow Cytometry (FC/FACS)
(Figure: FACS analysis using MAb to to Toll-like Receptor 4/MD-2 (mouse). Method: Mouse BaF3 cells (5x105) expressing surface mTLR4 alone or together with the accessory protein MD-2 or BaF3 parental cells, were incubated on ice for 30min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to Toll-like Receptor 4/MD-2 (mouse). After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.)
Rat anti-Mouse TLR4/MD-2 Complex Monoclonal Antibody | anti-TLR4 antibody
TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2)
95% (SDS-PAGE).
95% (SDS-PAGE).
Flow Cytometry (FC/FACS)
(Figure: FACS analysis using MAb to to Toll-like Receptor 4/MD-2 (mouse). Method: Mouse BaF3 cells (5x105) expressing surface mTLR4 alone or together with the accessory protein MD-2 or BaF3 parental cells, were incubated on ice for 30min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to Toll-like Receptor 4/MD-2 (mouse). After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(Figure: FACS analysis using MAb to to Toll-like Receptor 4/MD-2 (mouse). Method: Mouse BaF3 cells (5x105) expressing surface mTLR4 alone or together with the accessory protein MD-2 or BaF3 parental cells, were incubated on ice for 30min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to Toll-like Receptor 4/MD-2 (mouse). After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.)
Flow Cytometry (FC/FACS)
(Figure: Detection of endogenous mouse TLR4 by FACS analysis using the MAb to TLR4/MD-2 (mouse) (ATTO 488). Method: WEHI cells were stained with MAb to TLR4/MD-2 (mouse) labeled with FITC or with the new ATTO 488 dye. An isotype control labeled with FITC was used as reference.)
Flow Cytometry (FC/FACS)
(Figure: Detection of endogenous mouse TLR4 by FACS analysis using the MAb to TLR4/MD-2 (mouse) (ATTO 488). Method: WEHI cells were stained with MAb to TLR4/MD-2 (mouse) labeled with FITC or with the new ATTO 488 dye. An isotype control labeled with FITC was used as reference.)
NCBI and Uniprot Product Information
Uniprot Description
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().
Research Articles on TLR4
Similar Products
Product Notes
The TLR4 (Catalog #AAA600420) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TLR4/MD-2 Complex can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Flow Cytometry, Immunoprecipitation, Functional Application (inhibition of LPS-induced cytokine production; use preservative free formulation). Researchers should empirically determine the suitability of the TLR4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR4/MD-2 Complex, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
