Rat anti-Mouse TLR4/MD-2 Complex Monoclonal Antibody | anti-TLR4 antibody

TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2)

Cat. Num. AAA600997

• Reactivity: Mouse
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified IgG
• Synonyms: TLR4/MD-2 Complex; Monoclonal Antibody; TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2); Anti -TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2); anti-TLR4 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6A641
• Specificity: Reacts preferentially, especially in flow cytometry, with mouse TLR4 that is associated with MD-2. Recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 alone.
• Purity/Purification: Affinity Purified; Purified IgG
• Form/Format: Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

• Applicable Applications for anti-TLR4 antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Can be used for Flow Cytometry and Immunoprecipitation. The antibody recognizes the mouse TLR4/MD-2 complex and is not useful for detection of TLR4 alone. Furthermore the antibody can be used as antagonist for TLR4, it blocks LPS-induced cytokine production.; Dilution: Flow Cytometry: 1:10. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TLR4 antibody

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. This is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to coprecipitate MD-2 (30kD) with TLR4 (100kD).

Product Categories/Family for anti-TLR4 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 153792596
• NCBI GeneID: 100066890
• NCBI Accession #: NP_001093239.1
• NCBI GenBank Nucleotide #: NM_001099769.1
• UniProt Accession #: Q9MYW3
• NCBI Official Full Name: toll-like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• Protein Family: Toll-like receptor
• UniProt Entry Name: TLR4_HORSE

Uniprot Description

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().