Mouse anti-Human TLR1 Monoclonal Antibody | anti-TLR1 antibody

TLR1, Human, mAb GD2.F4, FITC

Cat. Num. AAA584339

• Gene Names: TLR1; TIL; CD281; rsc786; TIL. LPRS5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: TLR1; Monoclonal Antibody; Human; mAb GD2.F4; FITC; CD281; toll-like receptor 1; TLR1Fc; anti-TLR1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 1% bovine serum albumin.
• Sequence Length: 786

• Applicable Applications for anti-TLR1 antibody: F, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunofluorescence (IF), P
• Application Notes: Application Notes: FC: Antibody GD2.F4 stains the extracellular domain of TLR1. Monocytes were resuspended in PBS, 0.1% BSA, 0.02% NAN3 containing 15 ug/ml GD2.F4. As negative control an IgG1 isotype control was used (Ref.1).; Application Use: For flow cytometry and immunohistochemistry dilutions to be used depend on detection system
• Conjugate: FITC
• Immunogen: HeLa cells transfected with TLR1 mRNA (Ref.1)
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-TLR1 antibody

Product Description: The monoclonal antibody GD2.F4 reacts with human TLR1. Toll-like receptors (TLR) are highly conserved throughout evolution and play an essential role in recognizing conserved motifs found in various pathogens and initiating an appropriate innate immune response. In human, ten members of the TLR family have been identified as type I transmembrane signaling receptors containing multiple copies of leucine rich repeats in the extracellular domain and an interleukin-1 (IL-1) receptor motif in the cytoplasmic domain. Mammalian responsiveness to microbial products may be mediated by combinations of TLRs, for example a co-operative effect is observed between TLR1 and TLR2- in response to bacterial lipoproteins. On the other hand, TLR 1 was shown to have the capacity to abrogate TLR4 signaling. In general, TLR1 is expressed at higher levels as compared to other TLRs. The highest expression of TLR1 is found in monocytes but it can also be expressed by macrophages, dendritic cells, B, T, and NK cells. In recent studies, several human TLR1 polymorphisms have been associated with impaired mycobacterial signaling and susceptibility to tuberculosis.

References

1. Wyllie, D et al; Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterial responses. J Immunol 2000, 165: 7125
2. Visintin, A et al; Regulation of Toll-like receptors in human monocytes and dendritic cells. J Immunol 2001, 166: 249
3. Sandor, F et al; Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkB signaling. JCB 2003, 162: 1099
4. Ochoa, M et al; Distribution of Toll-like receptor 1 and Toll-like receptor 2 in human lymphoid tissue. Immunology 2003, 108: 10
5. Ritter, M et al; Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response. J Inflamm 2005, 2: 16
6. M¥nsson, A et al; A distinct Toll-like receptor repertoire in human tonsillar B cells, directly activated by Pam3CSK4, R-837 and CpG-2006 stimulation. Immunology 2006, 118: 539
7. Panda, A et al; Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. J Immunol 2010, 184: 2518

NCBI and Uniprot Product Information

• NCBI GI #: 41350337
• NCBI GeneID: 7096
• NCBI Accession #: NP_003254.2
• NCBI GenBank Nucleotide #: NM_003263.3
• Molecular Weight: 90,291 Da
• NCBI Official Full Name: toll-like receptor 1
• NCBI Official Synonym Full Names: toll-like receptor 1
• NCBI Official Symbol: TLR1
• NCBI Official Synonym Symbols: TIL; CD281; rsc786; TIL. LPRS5
• NCBI Protein Information: toll-like receptor 1; toll/interleukin-1 receptor-like protein
• UniProt Protein Name: Toll-like receptor 1
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR1
• UniProt Synonym Gene Names: KIAA0012; TIL
• UniProt Entry Name: TLR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR1: Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Binds MYD88 (via TIR domain). Interacts with CNPY3. Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2. Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity

Biological Process: MyD88-dependent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; regulation of cytokine secretion; macrophage activation; positive regulation of interleukin-6 biosynthetic process; innate immune response; immune response; detection of triacylated bacterial lipoprotein; inflammatory response; signal transduction; toll-like receptor 1 signaling pathway

Disease: Leprosy, Susceptibility To, 5