Mouse TLN1 / Talin Monoclonal Antibody | anti-TLN1 antibody

Mouse Monoclonal [clone 2E10] (IgG3,k) to Human TLN1 / Talin

Cat. Num. AAA246328

• Gene Names: TLN1; TLN; ILWEQ
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: TLN1 / Talin; Monoclonal Antibody; Mouse Monoclonal [clone 2E10] (IgG3; k) to Human TLN1 / Talin; Anti-TLN1 / Talin Antibody (clone 2E10) IHC-plus; TLN1; KIAA1027; TLN; Talin-1; ILWEQ; Talin; Talin 1; Human TLN1; anti-TLN1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 2E10
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Sequence Length: 2541

• Applicable Applications for anti-TLN1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control Raw 264. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: TLN1 (NP_006280, 1052 a.a. ~ 1150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: TLN1 / Talin antibody was raised against tLN1 (NP_006280, 1052 a.a. ~ 1150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-TLN1 / Talin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TLN1 / Talin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(TLN1 monoclonal antibody clone 2E10. Western blot of TLN1 expression in Raw 264.7.)

Western Blot (WB)

(TLN1 monoclonal antibody clone 2E10. Western blot of TLN1 expression in PC-12.)

ELISA (EIA)

(Detection limit for recombinant GST tagged TLN1 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 223029410
• NCBI GeneID: 7094
• NCBI Accession #: NP_006280.3
• NCBI GenBank Nucleotide #: NM_006289.3
• UniProt Accession #: Q9Y490; Q86YD0; Q9NZQ2; Q9UHH8; Q9UPX3; A6NMY0
• Molecular Weight: 269,767 Da
• NCBI Official Full Name: talin-1
• NCBI Official Synonym Full Names: talin 1
• NCBI Official Symbol: TLN1
• NCBI Official Synonym Symbols: TLN; ILWEQ
• NCBI Protein Information: talin-1
• UniProt Protein Name: Talin-1
• Protein Family: Talin
• UniProt Gene Name: TLN1
• UniProt Synonym Gene Names: KIAA1027; TLN
• UniProt Entry Name: TLN1_HUMAN

NCBI Description

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

Uniprot Description

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: ruffle; cell surface; focal adhesion; cytoplasm; extracellular region; plasma membrane; microtubule organizing center; intercellular junction; cytosol

Molecular Function: integrin binding; actin filament binding; protein binding; LIM domain binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding

Biological Process: platelet activation; axon guidance; intercellular junction assembly; unfolded protein response; cytoskeletal anchoring; cellular protein metabolic process; platelet degranulation; unfolded protein response, activation of signaling protein activity; muscle contraction; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; blood coagulation; cell motility